Dorsal skinfold chamber technique for intravital microscopy in nude mice.

Details

Serval ID
serval:BIB_CA557518E782
Type
Article: article from journal or magazin.
Collection
Publications
Title
Dorsal skinfold chamber technique for intravital microscopy in nude mice.
Journal
American Journal of Pathology
Author(s)
Lehr H.A., Leunig M., Menger M.D., Nolte D., Messmer K.
ISSN
0002-9440 (Print)
ISSN-L
0002-9440
Publication state
Published
Issued date
1993
Volume
143
Number
4
Pages
1055-1062
Language
english
Abstract
For many years, observation chambers, implanted in various animal species and in man, have been used for intravital microscopy of the microcirculation in granulation tissue, preformed tissue, or of the microvascularization of tissue implants. We describe herein a skinfold chamber model for the intravital microscopic investigation of striated skin muscle in immunoincompetent, nude mice over a minimum period of 2 weeks. Using fluorescent markers for contrast enhancement of plasma (fluorescein isothiocyanate-dextran) and leukocytes (acridine orange), the presented model allows the quantitative analysis of 1) the microhemodynamic parameters microvessel diameter and red blood cell velocity in arterioles (16 to 50 mu diameter), capillaries (4 to 9 mu diameter), and post-capillary venules (19 to 60 mu diameter), 2) leukocyte/endothelium interaction in these vessel segments, 3) functional capillary density and intercapillary distance, and 4) endothelial cell integrity. These parameters can be assessed in the microcirculation of the striated muscle tissue under normal or pathological conditions, as well as in the microcirculation of transplanted xenogeneic (human) neoplastic and non-neoplastic tissue grafts.
Keywords
Animals, Blood Vessels/anatomy & histology, Dextrans, Fluorescein-5-isothiocyanate/analogs & derivatives, Mice, Mice, Nude, Microcirculation, Microscopy, Fluorescence/methods, Muscles/blood supply, Skin Window Technique
Pubmed
Create date
25/11/2011 19:10
Last modification date
20/08/2019 15:45
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