Deleterious impact of a virulent bacteriophage on survival and biocontrol activity of Pseudomonas fluorescens strain CHAO in natural soil.

Détails

ID Serval
serval:BIB_C7B4FB4152AC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Deleterious impact of a virulent bacteriophage on survival and biocontrol activity of Pseudomonas fluorescens strain CHAO in natural soil.
Périodique
Molecular Plant-microbe Interactions
Auteur(s)
Keel C., Ucurum Z., Michaux P., Adrian M., Haas D.
ISSN
0894-0282 (Print)
ISSN-L
0894-0282
Statut éditorial
Publié
Date de publication
2002
Volume
15
Numéro
6
Pages
567-576
Langue
anglais
Résumé
Many biotic and abiotic factors affect the persistence and activity of beneficial pseudomonads introduced into soil to suppress plant diseases. One such factor may be the presence of virulent bacteriophages that decimate the population of the introduced bacteria, thereby reducing their beneficial effect. We have isolated a lytic bacteriophage (phi)GP100) that specifically infects the biocontrol bacterium Pseudomonas fluorescens CHA0 and some closely related Pseudomonas strains. phiGP100 was found to be a double-stranded-DNA phage with an icosahedral head, a stubby tail, and a genome size of approximately 50 kb. Replication of phiGP100 was negatively affected at temperatures higher than 25 degrees C. phiGP100 had a negative impact on the population size and the biocontrol activity of P. fluorescens strain CHA0-Rif (a rifampicin-resistant variant of CHA0) in natural soil microcosms. In the presence of phiGP100, the population size of strain CHA0-Rif in soil and on cucumber roots was reduced more than 100-fold. As a consequence, the bacterium's capacity to protect cucumber against a root disease caused by the pathogenic oomycete Pythium ultimum was entirely abolished. In contrast, the phage affected neither root colonization and nor the disease suppressive effect of a phiDGP100-resistant variant of strain CHA0-Rif. To our knowledge, this study is the first to illustrate the potential of phages to impair biocontrol performance of beneficial bacteria released into the natural soil environment.
Mots-clé
Bacteriophages/genetics, Bacteriophages/pathogenicity, Chloroform/pharmacology, Cucumis sativus/genetics, Cucumis sativus/microbiology, DNA, Viral/genetics, Hot Temperature, Immunity, Innate/genetics, Microscopy, Electron, Pest Control, Biological/methods, Plant Diseases/microbiology, Plant Roots/microbiology, Pseudomonas fluorescens/genetics, Pseudomonas fluorescens/virology, Soil Microbiology, Ultraviolet Rays, Virulence, Virus Replication/drug effects, Virus Replication/radiation effects
Pubmed
Web of science
Création de la notice
24/01/2008 14:51
Dernière modification de la notice
03/03/2018 21:20
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