Genome analysis of a major urban malaria vector mosquito, Anopheles stephensi.

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Etat: Serval
Version: Final published version
ID Serval
serval:BIB_C5AFD9D10938
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Genome analysis of a major urban malaria vector mosquito, Anopheles stephensi.
Périodique
Genome Biology
Auteur(s)
Jiang X., Peery A., Hall A.B., Sharma A., Chen X.G., Waterhouse R.M., Komissarov A., Riehle M.M., Shouche Y., Sharakhova M.V., Lawson D., Pakpour N., Arensburger P., Davidson V.L., Eiglmeier K., Emrich S., George P., Kennedy R.C., Mane S.P., Maslen G., Oringanje C., Qi Y., Settlage R., Tojo M., Tubio J.M., Unger M.F., Wang B., Vernick K.D., Ribeiro J.M., James A.A., Michel K., Riehle M.A., Luckhart S., Sharakhov I.V., Tu Z.
ISSN
1474-760X (Electronic)
ISSN-L
1474-7596
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
15
Numéro
9
Pages
459
Langue
anglais
Résumé
Anopheles stephensi is the key vector of malaria throughout the Indian subcontinent and Middle East and an emerging model for molecular and genetic studies of mosquito-parasite interactions. The type form of the species is responsible for the majority of urban malaria transmission across its range.
Here, we report the genome sequence and annotation of the Indian strain of the type form of An. stephensi. The 221 Mb genome assembly represents more than 92% of the entire genome and was produced using a combination of 454, Illumina, and PacBio sequencing. Physical mapping assigned 62% of the genome onto chromosomes, enabling chromosome-based analysis. Comparisons between An. stephensi and An. gambiae reveal that the rate of gene order reshuffling on the X chromosome was three times higher than that on the autosomes. An. stephensi has more heterochromatin in pericentric regions but less repetitive DNA in chromosome arms than An. gambiae. We also identify a number of Y-chromosome contigs and BACs. Interspersed repeats constitute 7.1% of the assembled genome while LTR retrotransposons alone comprise more than 49% of the Y contigs. RNA-seq analyses provide new insights into mosquito innate immunity, development, and sexual dimorphism.
The genome analysis described in this manuscript provides a resource and platform for fundamental and translational research into a major urban malaria vector. Chromosome-based investigations provide unique perspectives on Anopheles chromosome evolution. RNA-seq analysis and studies of immunity genes offer new insights into mosquito biology and mosquito-parasite interactions.

Mots-clé
Animals, Anopheles/genetics, Anopheles/metabolism, Chromosome Mapping, Chromosomes, Insect/genetics, Cluster Analysis, Evolution, Molecular, Genome, Insect, Humans, Insect Proteins/genetics, Insect Proteins/metabolism, Insect Vectors/genetics, Malaria/transmission, Phylogeny, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Synteny, Transcriptome, Urban Population
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/09/2017 10:56
Dernière modification de la notice
09/05/2019 1:00
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