PCR detection of bacteria on cardiac valves of patients with treated bacterial endocarditis
Details
Serval ID
serval:BIB_C5A894BA0FE8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
PCR detection of bacteria on cardiac valves of patients with treated bacterial endocarditis
Journal
Journal of Clinical Microbiology
ISSN
0095-1137 (Print)
Publication state
Published
Issued date
01/2005
Volume
43
Number
1
Pages
163-7
Notes
Journal Article --- Old month value: Jan
Abstract
We used broad-range PCR amplification and sequencing to detect and identify bacterial DNA in 156 valves of patients treated for infective endocarditis (IE). Bacterial DNA was found more frequently in patients who underwent valve replacement while on antibiotic treatment for IE (60%) than in patients who had completed antibiotic treatment for IE (37%; P = 0.02). We found specific bacterial DNA in valves removed from 11 of 30 patients who had completed antibiotic treatment for IE. Six had no histological evidence of IE. The presence of DNA was significantly correlated with the presence of histologic lesions (P = 0.001) and with the presence of bacteria detected by Gram staining (P < 0.001). Bartonella and streptococci were detected for much longer after antibiotic treatment by PCR than other species (P = 0.047 and 0.04, respectively), and coagulase-negative staphylococci were detected for much shorter periods (P = 0.02). The finding that bacterial DNA was more likely to be detected in valves of patients with active IE than in patients who had completed antibiotic treatment for IE shows that bacterial DNA is cleared slowly. There was no significant correlation between the duration of antibiotic therapy and the presence of bacterial DNA in valves. Since the persistence of bacterial DNA in valves does not necessarily indicate the persistence of viable bacteria, the detection of bacterial DNA in valves from IE patients should be interpreted with caution, in particular in those patients with a past history of treated IE.
Keywords
Adult
Aged
Aged, 80 and over
Anti-Bacterial Agents/*therapeutic use
Bartonella/genetics/*isolation & purification
DNA, Bacterial/analysis
Endocarditis, Bacterial/*drug therapy/microbiology
Female
Gram-Positive Cocci/genetics/*isolation & purification
Heart Valve Diseases/microbiology
Heart Valve Prosthesis/microbiology
Heart Valve Prosthesis Implantation
Heart Valves/*microbiology
Humans
Male
Middle Aged
Polymerase Chain Reaction/*methods
Prospective Studies
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:28
Last modification date
20/08/2019 16:41