Improved multiple-locus variable-number tandem-repeat assay for Staphylococcus aureus genotyping, providing a highly informative technique together with strong phylogenetic value.

Détails

ID Serval
serval:BIB_C54BAECDBFFB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Improved multiple-locus variable-number tandem-repeat assay for Staphylococcus aureus genotyping, providing a highly informative technique together with strong phylogenetic value.
Périodique
Journal of clinical microbiology
Auteur(s)
Pourcel C., Hormigos K., Onteniente L., Sakwinska O., Deurenberg R.H., Vergnaud G.
ISSN
1098-660X[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
47
Numéro
10
Pages
3121-3128
Langue
anglais
Notes
Publication types: Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
We describe an improved multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) scheme for genotyping Staphylococcus aureus. We compare its performance to those of multilocus sequence typing (MLST) and spa typing in a survey of 309 strains. This collection includes 87 epidemic methicillin-resistant S. aureus (MRSA) strains of the Harmony collection, 75 clinical strains representing the major MLST clonal complexes (CCs) (50 methicillin-sensitive S. aureus [MSSA] and 25 MRSA), 135 nasal carriage strains (133 MSSA and 2 MRSA), and 13 published S. aureus genome sequences. The results show excellent concordance between the techniques' results and demonstrate that the discriminatory power of MLVA is higher than those of both MLST and spa typing. Two hundred forty-two genotypes are discriminated with 14 VNTR loci (diversity index, 0.9965; 95% confidence interval, 0.9947 to 0.9984). Using a cutoff value of 45%, 21 clusters are observed, corresponding to the CCs previously defined by MLST. The variability of the different tandem repeats allows epidemiological studies, as well as follow-up of the evolution of CCs and the identification of potential ancestors. The 14 loci can conveniently be analyzed in two steps, based upon a first-line simplified assay comprising a subset of 10 loci (panel 1) and a second subset of 4 loci (panel 2) that provides higher resolution when needed. In conclusion, the MLVA scheme proposed here, in combination with available on-line genotyping databases (including http://mlva.u-psud.fr/), multiplexing, and automatic sizing, can provide a basis for almost-real-time large-scale population monitoring of S. aureus.
Mots-clé
Bacterial Typing Techniques/methods, Carrier State/microbiology, Cluster Analysis, DNA Fingerprinting/methods, DNA, Bacterial/genetics, Genotype, Humans, Minisatellite Repeats, Molecular Epidemiology/methods, Phylogeny, Sensitivity and Specificity, Staphylococcal Infections/microbiology, Staphylococcus aureus/classification, Staphylococcus aureus/isolation & purification
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/02/2010 15:26
Dernière modification de la notice
09/05/2019 0:59
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