An intact small animal model of myocardial ischemia-reperfusion: Characterization of metabolic changes by hyperpolarized 13C MR spectroscopy

Détails

ID Serval
serval:BIB_C44C567CF402
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An intact small animal model of myocardial ischemia-reperfusion: Characterization of metabolic changes by hyperpolarized 13C MR spectroscopy
Périodique
American Journal of Physiology. Heart and Circulatory Physiology
Auteur(s)
Yoshihara H.A., Bastiaansen J.A., Berthonneche C., Comment A., Schwitter J.
ISSN
1522-1539 (Electronic)
ISSN-L
0363-6135
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
309
Numéro
12
Pages
H2058-H2066
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Hyperpolarized carbon-13 magnetic resonance spectroscopy ((13)C MRS) enables the sensitive and noninvasive assessment of the metabolic changes occurring during myocardial ischemia-reperfusion. Ischemia-reperfusion models using hyperpolarized (13)C MRS are established in heart preparations ex vivo and in large animals in vivo, but an in vivo model in small animals would be advantageous to allow the study of reperfusion metabolism with neuroendocrine and inflammatory responses intact with the option to perform a greater number of experiments. A novel intact rat model of ischemia-reperfusion is presented that incorporates hyperpolarized (13)C MRS to characterize reperfusion metabolism. Typically, in an in vivo model, a tissue input function (TIF) is required to account for apparent changes in the metabolism of injected hyperpolarized [1-(13)C]pyruvate resulting from changes in perfusion. Whereas the measurement of a TIF by metabolic imaging is particularly challenging in small animals, the ratios of downstream metabolites can be used as an alternative. The ratio of [(13)C]bicarbonate:[1-(13)C]lactate (RatioBic/Lac) measured within 1-2 min after coronary release decreased vs. baseline in ischemic rats (n = 10, 15-min occlusion, controls: n = 10; P = 0.017 for interaction, 2-way ANOVA). The decrease in oxidative pyruvate metabolism [RatioBic/Lac(Ischemia)/RatioBic/Lac(Baseline)] modestly correlated with area at risk (r = 0.66; P = 0.002). Hyperpolarized (13)C MRS was also used to examine alanine production during ischemia, which is observed in ex vivo models, but no significant change was noted; metrics incorporating [1-(13)C]alanine did not substantially improve the discrimination of ischemic-reperfused myocardium from nonischemic myocardium. This intact rat model, which mimics the human situation of reperfused myocardial infarction, could be highly valuable for the testing of new drugs to treat reperfusion injury, thereby facilitating translational research.
Mots-clé
Alanine/metabolism, Bicarbonates/metabolism, Inflammation/metabolism, Inflammation/pathology, Lactates/metabolism, Magnetic Resonance Spectroscopy/methods, Myocardial Reperfusion Injury/metabolism, Myocardial Reperfusion Injury/pathology, Neurosecretory Systems/metabolism, Neurosecretory Systems/pathology, Pyruvates/metabolism
Pubmed
Web of science
Création de la notice
11/10/2016 16:29
Dernière modification de la notice
20/08/2019 16:39
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