A new epitope of the T200 molecule family defined by the 3A35 monoclonal antibody and expressed by macrophages and activated T lymphocytes

Détails

ID Serval
serval:BIB_C4022E6B9FA6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A new epitope of the T200 molecule family defined by the 3A35 monoclonal antibody and expressed by macrophages and activated T lymphocytes
Périodique
European Journal of Immunology
Auteur(s)
Le Corre  R., Gerlier  D., Martin  A., Le Garrec  Y., Corradin  G., Bron  C., Toujas  L.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
03/1987
Volume
17
Numéro
3
Pages
327-33
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
A monoclonal antibody (mAb), 3A35, produced against mouse macrophages (M phi) was found to react against certain activated T cells. This mAb, a rat IgM, resulted from a cell fusion between a mouse plasmacytoma and rat lymphocytes immunized against mouse M phi. It bound more avidly to activated than to resident M phi. It did not react against B cells and resting T lymphocytes but recognized certain dividing T cells like EL4 lymphoma, concanavalin A-activated and interleukin 2-expanded spleen cells, and helper T cell hybridomas. By contrast, other T lymphocyte-derived cell lines such as YAC-1 and CTLL2 were unreactive. No clear relationship was found between the binding of 3A35 to cells and the expression of L3T4 and Lyt-2 antigens. The specific stimulation of T cell clones with antigen rapidly induced a strong reactivity with 3A35 mAb which declined thereafter to a low (helper clones) or non-reactivity (cytotoxic clones) after 10 days of culture. Immunoprecipitation experiments, performed with M phi derived from bone marrow cell cultures, surface iodinated with 125I or metabolically labeled with [35S]methionine, showed that 3A35 bound to a 200-kDa molecule, shifting to 175 kDa under reducing conditions. In peritoneal M phi activated in vivo, in addition to the 175-kDa band, new bands migrating at 140, 120 and 85 kDa were identified by 3A35 and could be absorbed on a commercial anti-T200 mAb bound to Sepharose beads. After strengthening the cell binding of 3A35 to EL4 lymphoma cells by a cross-linking agent, only a 85-kDa molecule was immunoprecipitated. Thus, 3A35 identifies a new epitope of the T200 molecule family which is expressed on M phi and activated T cells.
Mots-clé
Animals Antigens, CD45 Antigens, Surface/*immunology B-Lymphocytes/immunology Epitopes Flow Cytometry Histocompatibility Antigens/*immunology Immunologic Capping Lymphocyte Activation Macrophages/*immunology Mice Molecular Weight T-Lymphocytes/classification/cytology/*immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
03/03/2018 21:12
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