Microtubule-associated protein 1B binds glyceraldehyde-3-phosphate dehydrogenase.

Details

Serval ID
serval:BIB_C3B9F7663437
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Microtubule-associated protein 1B binds glyceraldehyde-3-phosphate dehydrogenase.
Journal
Journal of Proteome Research
Author(s)
Cueille N., Blanc C.T., Riederer I.M., Riederer B.M.
ISSN
1535-3893 (Print)
ISSN-L
1535-3893
Publication state
Published
Issued date
2007
Volume
6
Number
7
Pages
2640-2647
Language
english
Abstract
Microtubule-associated protein 1B, MAP1B, is a major cytoskeletal protein during brain development and one of the largest brain MAPs associated with microtubules and microfilaments. Here, we identified several proteins that bind to MAP1B via immunoprecipitation with a MAP1B-specific antibody, by one and two-dimensional gel electrophoresis and subsequent mass spectrometry identification of precipitated proteins. In addition to tubulin and actin, a variety of proteins were identified. Among these proteins were glyceraldehyde-3-phosphate dehydrogenase (GAPDH), heat shock protein 8, dihydropyrimidinase related proteins 2 and 3, protein-L-isoaspartate O-methyltransferase, beta-spectrin, and clathrin protein MKIAA0034, linking either directly or indirectly to MAP1B. In particular, GAPDH, a key glycolytic enzyme, was bound in large quantity to the heavy chain of MAP1B in adult brain tissue. In vitro binding studies confirmed a direct binding of GAPDH to MAP1B. In PC12 cells, GAPDH was found in cytoplasm and nuclei and partially co-localized with MAP1B. It disappeared from the cytoplasm under oxidative stress or after a disruption of cytoskeletal elements after colcemid or cytochalasin exposure. GAPDH may be essential in the local energy provision of cytoskeletal structures and MAP1B may help to keep this key enzyme close to the cytoskeleton.
Keywords
Animals, Brain/metabolism, Brain Chemistry, Cell Nucleus/chemistry, Cytoplasm/chemistry, Glyceraldehyde-3-Phosphate Dehydrogenases/analysis, Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism, Immunoprecipitation, Mice, Microtubule-Associated Proteins/analysis, Microtubule-Associated Proteins/metabolism, Nerve Tissue Proteins/analysis, Nerve Tissue Proteins/chemistry
Pubmed
Web of science
Create date
24/01/2008 14:35
Last modification date
20/08/2019 15:38
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