Article: article from journal or magazin.
Determination of trimipramine and its demethylated and hydroxylated metabolites in plasma by gas chromatography-mass spectrometry.
Journal of Chromatography
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
A gas chromatographic-mass spectrometric (GC-MS) method has been developed, for the determination of trimipramine (TRI), desmethyltrimipramine (DTRI), didesmethyltrimipramine (DDTRI), 2-hydroxytrimipramine (2-OH-TRI) and 2-hydroxydesmethyltrimipramine (2-OH-DTRI). The method includes two derivatization steps with trifluoroacetic acid anhydride and N-methyl-N-(tert.-butyldimethyl silyl)trifluoroacetamide and the use of an SE-54 capillary silica column. The limits of quantitation were found to be 2 ng/ml for DTRI and 4 ng/ml for all other substances. Besides, methods have been optimized for the hydrolysis of the glucuronic acid conjugated metabolites. This specific detection method is useful, as polymedication is a usual practice in clinical situations, and its sensitivity allows its use for single-dose pharmacokinetic studies.
Biotransformation, Chromatography, High Pressure Liquid, Dealkylation, Gas Chromatography-Mass Spectrometry, Glucuronates/metabolism, Humans, Hydroxylation, Trimipramine/blood, Trimipramine/pharmacokinetics
Web of science
Last modification date