Determination of trimipramine and its demethylated and hydroxylated metabolites in plasma by gas chromatography-mass spectrometry.

Détails

ID Serval
serval:BIB_C2C3D83016F6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Determination of trimipramine and its demethylated and hydroxylated metabolites in plasma by gas chromatography-mass spectrometry.
Périodique
Journal of Chromatography
Auteur(s)
Eap C.B., Koeb L., Baumann P.
ISSN
0021-9673 (Print)
ISSN-L
0021-9673
Statut éditorial
Publié
Date de publication
1994
Peer-reviewed
Oui
Volume
652
Numéro
1
Pages
97-103
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
A gas chromatographic-mass spectrometric (GC-MS) method has been developed, for the determination of trimipramine (TRI), desmethyltrimipramine (DTRI), didesmethyltrimipramine (DDTRI), 2-hydroxytrimipramine (2-OH-TRI) and 2-hydroxydesmethyltrimipramine (2-OH-DTRI). The method includes two derivatization steps with trifluoroacetic acid anhydride and N-methyl-N-(tert.-butyldimethyl silyl)trifluoroacetamide and the use of an SE-54 capillary silica column. The limits of quantitation were found to be 2 ng/ml for DTRI and 4 ng/ml for all other substances. Besides, methods have been optimized for the hydrolysis of the glucuronic acid conjugated metabolites. This specific detection method is useful, as polymedication is a usual practice in clinical situations, and its sensitivity allows its use for single-dose pharmacokinetic studies.
Mots-clé
Biotransformation, Chromatography, High Pressure Liquid, Dealkylation, Gas Chromatography-Mass Spectrometry, Glucuronates/metabolism, Humans, Hydroxylation, Trimipramine/blood, Trimipramine/pharmacokinetics
Pubmed
Web of science
Création de la notice
01/03/2013 11:25
Dernière modification de la notice
03/03/2018 21:10
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