The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis.

Details

Serval ID
serval:BIB_C29E08F5343D
Type
Article: article from journal or magazin.
Collection
Publications
Title
The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis.
Journal
Nature Cell Biology
Author(s)
Huang Q., Gumireddy K., Schrier M., le Sage C., Nagel R., Nair S., Egan D.A., Li A., Huang G., Klein-Szanto A.J., Gimotty P.A., Katsaros D., Coukos G., Zhang L., Puré E., Agami R.
ISSN
1476-4679 (Electronic)
ISSN-L
1465-7392
Publication state
Published
Issued date
2008
Volume
10
Number
2
Pages
202-210
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
MicroRNAs (miRNAs) are single-stranded, noncoding RNAs that are important in many biological processes. Although the oncogenic and tumour-suppressive functions of several miRNAs have been characterized, the role of miRNAs in mediating tumour metastasis was addressed only recently and still remains largely unexplored. To identify potential metastasis-promoting miRNAs, we set up a genetic screen using a non-metastatic, human breast tumour cell line that was transduced with a miRNA-expression library and subjected to a trans-well migration assay. We found that human miR-373 and miR-520c stimulated cancer cell migration and invasion in vitro and in vivo, and that certain cancer cell lines depend on endogenous miR-373 activity to migrate efficiently. Mechanistically, the migration phenotype of miR-373 and miR-520c can be explained by suppression of CD44. We found significant upregulation of miR-373 in clinical breast cancer metastasis samples that correlated inversely with CD44 expression. Taken together, our findings indicate that miRNAs are involved in tumour migration and invasion, and implicate miR-373 and miR-520c as metastasis-promoting miRNAs.
Keywords
Animals, Antigens, CD44/genetics, Antigens, CD44/metabolism, Breast Neoplasms/metabolism, Breast Neoplasms/pathology, Cell Line, Tumor, Cell Migration Assays, Cell Movement/physiology, Colonic Neoplasms/metabolism, Colonic Neoplasms/pathology, Female, Humans, Lymphatic Metastasis, Male, Mice, Mice, SCID, MicroRNAs/biosynthesis, MicroRNAs/physiology, Neoplasm Invasiveness/genetics, Neoplasm Invasiveness/pathology, Neoplasm Metastasis/genetics, Neoplasm Metastasis/pathology, Neoplasm Transplantation, Prostatic Neoplasms/metabolism, Prostatic Neoplasms/pathology, Transplantation, Heterologous
Pubmed
Web of science
Create date
14/10/2014 11:42
Last modification date
20/08/2019 15:37
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