Estrogen receptor level determines sex-specific in vitro transcription from the Xenopus vitellogenin promoter.

Details

Serval ID
serval:BIB_C20236845AE4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Estrogen receptor level determines sex-specific in vitro transcription from the Xenopus vitellogenin promoter.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Corthésy B., Claret F.X., Wahli W.
ISSN
0027-8424[print], 0027-8424[linking]
Publication state
Published
Issued date
10/1990
Volume
87
Number
20
Pages
7878-7882
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Female-specific expression of the Xenopus laevis vitellogenin gene was reconstituted in vitro by addition of recombinant vaccinia-virus-produced estrogen receptor to nuclear extracts from male livers, in which this gene is silent. Transcription enhancement was at least 30 times and was selectively restricted to vitellogenin templates containing the estrogen-responsive unit. Thus, in male hepatocytes, estrogen receptor is the limiting regulatory factor that in the female liver controls efficient and accurate sex-specific expression of the vitellogenin gene. Furthermore, the Xenopus liver factor B, which is essential in addition to the estrogen receptor for the activation of this gene, was successfully replaced in the Xenopus extract by purified human nuclear factor I, identifying factor B of Xenopus as a functional homolog of this well-characterized human transcription factor.
Keywords
Animals, Base Sequence, CCAAT-Enhancer-Binding Proteins, Cell Nucleus/metabolism, Chromosome Deletion, DNA-Binding Proteins/metabolism, Female, Humans, Male, Molecular Sequence Data, Mutagenesis, Site-Directed, NFI Transcription Factors, Nuclear Proteins, Oligonucleotide Probes, Promoter Regions, Genetic, Receptors, Estrogen/physiology, Restriction Mapping, Sex Factors, Templates, Genetic, Transcription Factors, Transcription, Genetic, Vitellogenins/genetics, Xenopus laevis, Y-Box-Binding Protein 1
Pubmed
Web of science
Create date
25/01/2008 14:53
Last modification date
20/08/2019 15:36
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