Complementary DNA sequence and chromosomal mapping of a human proteoglycan-binding cell-adhesion protein (dermatopontin).

Détails

ID Serval
serval:BIB_C0DD26D40E59
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Complementary DNA sequence and chromosomal mapping of a human proteoglycan-binding cell-adhesion protein (dermatopontin).
Périodique
Genomics
Auteur(s)
Superti-Furga A., Rocchi M., Schäfer B.W., Gitzelmann R.
ISSN
0888-7543 (Print)
ISSN-L
0888-7543
Statut éditorial
Publié
Date de publication
1993
Volume
17
Numéro
2
Pages
463-467
Langue
anglais
Résumé
We have noticed the presence of a protein with a M(r) of approx 22 kDa in proteoglycan preparations from human fibroblast cultures and speculated that it might be related to a 22-kDa protein from bovine skin (22K) with proteoglycan- and cell-binding properties. Using degenerated oligomers designed from the amino acid sequence of the bovine protein, we amplified and subcloned sequences from human fibroblast and fibrosarcoma cDNA. The three clones that were characterized contain an open reading frame (603 bp) coding for 201 amino acids comprising a secretory leader peptide of 18 amino acids and a secreted part of 183 amino acids with 96% identity to the bovine sequence, indicating that they code for the human homologue ("dermatopontin") of the bovine 22K protein. Expression of dermatopontin is not limited to connective tissue, as Northern blots show specific mRNAs in cultured fibroblasts, muscle, heart, pancreas, and lung. Two species of mRNA (1.0 and 2.2 kb) are present, indicating alternative polyadenylation or alternative splicing. The cDNA clones map to 1q12-q23 in a cell hybrid panel containing specific chromosomal deletions.
Mots-clé
Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Carrier Proteins/genetics, Carrier Proteins/isolation & purification, Cattle, Cell Adhesion Molecules/genetics, Cell Adhesion Molecules/isolation & purification, Cells, Cultured, Chondroitin Sulfate Proteoglycans, Chromosome Mapping, Chromosomes, Human, Pair 1, Cloning, Molecular, DNA, Complementary/genetics, Extracellular Matrix Proteins, Fibroblasts/metabolism, Humans, Hybrid Cells, Molecular Sequence Data, Molecular Weight, Oligodeoxyribonucleotides, Open Reading Frames, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, RNA, Messenger/biosynthesis, RNA, Messenger/isolation & purification, Sequence Homology, Amino Acid, Skin/metabolism
Pubmed
Web of science
Création de la notice
14/03/2011 17:14
Dernière modification de la notice
03/03/2018 21:06
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