Quantitative assessment of cell viability and apoptosis in cultured epidermal autografts: application to burn therapy.

Détails

ID Serval
serval:BIB_C0B9D0FD04DD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Quantitative assessment of cell viability and apoptosis in cultured epidermal autografts: application to burn therapy.
Périodique
International Journal of Artificial Organs
Auteur(s)
Vernez M., Raffoul W., Gailloud-Matthieu M.C., Egloff D., Senechaud I., Panizzon R.G., Benathan M.
ISSN
0391-3988
Statut éditorial
Publié
Date de publication
09/2003
Peer-reviewed
Oui
Volume
26
Numéro
9
Pages
793-803
Langue
anglais
Notes
Publication types: Journal Article - Publication Status: ppublish
Résumé
Cultured epidermal autografts (CEA) have been used in the treatment of burns for almost two decades but the clinical results are still inconsistent. In a group of 37 patients with extensive burn wounds admitted to the University Hospital of Lausanne, CEA take ranged between 10 and 100% with a mean of 65%. To investigate CEA efficacy in burns, twelve CEA preparations were tested for their biological properties with particular emphasis on the balance between cell viability and apoptosis. Apoptosis was evaluated by in situ end-labeling (TUNEL), detection of DNA fragments in CEA extracts and analysis of caspase-3 activity. All CEA samples displayed a high cell viability (> 90%) and a low apoptosis rate (< 6%). However, several biological parameters including the activity of transglutaminase showed wide interindividual variability suggesting that CEA therapeutic efficacy could be partly determined by intrinsic biological factors.
Mots-clé
Adolescent, Adult, Apoptosis, Burns, Cell Survival, Cells, Cultured, Child, Child, Preschool, DNA Fragmentation, Female, Humans, In Situ Nick-End Labeling, Infant, Keratinocytes, Male, Middle Aged, Skin Transplantation, Transglutaminases, Transplantation, Autologous, Treatment Outcome, Wound Healing
Pubmed
Web of science
Création de la notice
25/01/2008 17:54
Dernière modification de la notice
03/03/2018 21:06
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