Detection of colistin-resistant Gram-negative rods by using the SuperPolymyxin medium.

Détails

ID Serval
serval:BIB_BFE2CA09660D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detection of colistin-resistant Gram-negative rods by using the SuperPolymyxin medium.
Périodique
Diagnostic microbiology and infectious disease
Auteur(s)
Jayol A., Poirel L., André C., Dubois V., Nordmann P.
ISSN
1879-0070 (Electronic)
ISSN-L
0732-8893
Statut éditorial
Publié
Date de publication
10/2018
Peer-reviewed
Oui
Volume
92
Numéro
2
Pages
95-101
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The commercial SuperPolymyxin medium (ELITechGroup, Puteaux, France), currently licensed in Europe, is aimed to screen for either intrinsic or acquired colistin resistance in Gram negatives. It was tested for screening colistin-resistant Gram-negative rods from bacterial cultures of 145 colistin-resistant and 86 colistin-susceptible Gram-negative rods isolates. It exhibited a sensitivity and specificity of 95.2% (138/145) and 95.3% (82/86), respectively. The sensitivity for the detection of the 25 isolates carrying plasmid-mediated colistin resistance genes (mcr-1, mcr-2, mcr-3, and mcr-4) from bacterial cultures was of 100%. It was also evaluated for the detection of colistin-resistant Gram-negative rods from 57 rectal swab samples (41 clinical rectal swab samples and from 16 spiked rectal swab samples supplemented with colistin-resistant enterobacterial isolates carrying mcr genes). The overall sensitivity and specificity were of 100% (33/33) and 90.3% (56/62), respectively. The detection of isolates carrying plasmid-mediated mcr-like genes from rectal swabs was achieved with a lowest detection limit of 10 <sup>3</sup> to 10 <sup>4</sup>  CFU/mL. The SuperPolymyxin medium is adapted for screening and detection of colistin-resistant isolates from bacterial cultures and rectal swab samples, regardless of the level and the mechanism of colistin resistance. It might be useful in the context of prevention of outbreaks due to colistin-resistant isolates and for epidemiological surveillance.
Mots-clé
Anti-Bacterial Agents/pharmacology, Colistin/pharmacology, Culture Media, Drug Resistance, Bacterial, Enterobacteriaceae/drug effects, Enterobacteriaceae/isolation & purification, Gram-Negative Bacteria/drug effects, Gram-Negative Bacteria/isolation & purification, Humans, Microbial Sensitivity Tests, Plasmids, Polymyxins, Reagent Kits, Diagnostic, Colistin, MCR, Resistance, SuperPolymyxin medium, Susceptibility testing
Pubmed
Web of science
Création de la notice
08/10/2018 17:34
Dernière modification de la notice
21/01/2019 7:11
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