Partial denervation of the whiskerpad in adult mice: pattern and origin of reinnervation

Details

Serval ID
serval:BIB_BFDDABF521CA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Partial denervation of the whiskerpad in adult mice: pattern and origin of reinnervation
Journal
European Journal of Neuroscience
Author(s)
Corthesy  M. E., Bronchti  G., Welker  E.
ISSN
0953-816X (Print)
Publication state
Published
Issued date
08/1999
Volume
11
Number
8
Pages
2835-46
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
We studied sensory organ reinnervation after nerve transection in the mouse whisker-to-barrel pathway. In one set of adult mice, we determined at light microscopy level the number of fibres reaching the caudal whisker follicles 5, 15, 20, 60, 100 days and 1 year after transection of the sensory nerve of row C. Regenerated fibres were first detected 15 days post lesionem (p.l.) and myelin first observed at 20 days. Between 60 and 100 days, the number of fibres stayed at approximately 80% of the values obtained in control animals. At that time, myelinated fibres reached only 58% of their number in controls. At the electron microscopy level, these fibres differ from control ones by a smaller fibre diameter. The innervation of follicles of adjacent rows was not modified, indicating that follicular reinnervation is row specific. We checked this feature by injecting in another set of mice the denervated follicles and the adjacent ones with distinct retrograde tracers 45 days and 1 year after nerve transection. The percentage of double-labelled neurons in the Gasserian ganglion did not increase in experimental animals. This confirms the absence of colonization of intact follicles by regenerating fibres and indicates that reinnervation of the whisker follicles takes place by regeneration of the degenerated axons without collateral reinnervation. The companion paper describes the pattern of activation of the barrel cortex relative to the present findings.
Keywords
Amidines Animals Denervation Fluorescent Dyes Hair Follicle/physiology/ultrastructure Mice Mice, Inbred ICR Microscopy, Electron Nerve Regeneration/*physiology Nervous System Physiology Vibrissae/*innervation/ultrastructure
Pubmed
Web of science
Create date
24/01/2008 14:41
Last modification date
20/08/2019 15:34
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