Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1.

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Serval ID
serval:BIB_BEA4A7B8F3DF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Production externe
Title
Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1.
Journal
Antonie van Leeuwenhoek
Author(s)
Itoh Y., Soldati L., Leisinger T., Haas D.
ISSN
0003-6072 (Print)
ISSN-L
0003-6072
Publication state
Published
Issued date
1988
Volume
54
Number
6
Pages
567-573
Language
english
Abstract
The cloning vector pME290 (6.8 kb), which is derived from the Pseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1-3 copies) and pME294 (about 15-20 copies) were isolated. These vectors were used in the characterization of the P. aeruginosa argF gene encoding ornithine carbamoyltransferase.
Keywords
Cloning, Molecular, DNA, Bacterial/ultrastructure, DNA, Recombinant/ultrastructure, Gene Expression Regulation, Genetic Vectors, Plasmids, Pseudomonas aeruginosa/genetics
DOI
10.1007/BF00588392
Pubmed
3148293
Web of science
A1988R424300008
Create date
25/01/2008 18:01
Last modification date
20/08/2019 16:32
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