Article: article from journal or magazin.
Low- and intermediate-copy-number cloning vectors based on the Pseudomonas plasmid pVS1.
Antonie van Leeuwenhoek
The cloning vector pME290 (6.8 kb), which is derived from the Pseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1-3 copies) and pME294 (about 15-20 copies) were isolated. These vectors were used in the characterization of the P. aeruginosa argF gene encoding ornithine carbamoyltransferase.
Cloning, Molecular, DNA, Bacterial/ultrastructure, DNA, Recombinant/ultrastructure, Gene Expression Regulation, Genetic Vectors, Plasmids, Pseudomonas aeruginosa/genetics
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