Typing of Listeria monocytogenes by restriction polymorphism of the ribosomal ribonucleic acid gene region

Détails

ID Serval
serval:BIB_BCD3C94F2B7A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Typing of Listeria monocytogenes by restriction polymorphism of the ribosomal ribonucleic acid gene region
Périodique
Zentralbl Bakteriol
Auteur(s)
Jacquet  C., Bille  J., Rocourt  J.
ISSN
0934-8840
Statut éditorial
Publié
Date de publication
02/1992
Peer-reviewed
Oui
Volume
276
Numéro
3
Pages
356-65
Notes
Journal Article --- Old month value: Feb
Résumé
Ninety four strains of Listeria monocytogenes of different serovars and phagovars as well as of varying origins were characterized by ribosomal RNA gene restriction polymorphism. After digestion by EcoRI or HindIII, chromosomal DNAs were hybridized with a cloned rDNA probe from Bacillus subtilis that included the 16S rRNA gene. The 94 strains were divided into 14 ribovars according to the different hybridization patterns generated by cleavage with EcoRI. Less important genomic heterogeneity could be detected when DNAs were digested by HindIII. EcoRI ribovars analysis allowed to describe a new typing scheme which did not corroborate routine typings such as serotyping and phage typing. It also confirmed a new view of this species in exhibiting a clone gathering most human strains, as first inferred from multilocus enzyme analysis (Piffaretti et al., 22).
Mots-clé
Animals Autoradiography Bacteriophage Typing DNA Probes DNA, Bacterial/*analysis Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Humans Listeria monocytogenes/*classification/genetics Nucleic Acid Hybridization *Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Restriction Mapping Serotyping
Pubmed
Web of science
Création de la notice
11/02/2008 13:39
Dernière modification de la notice
03/03/2018 20:57
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