Typing of Listeria monocytogenes by restriction polymorphism of the ribosomal ribonucleic acid gene region
Details
Serval ID
serval:BIB_BCD3C94F2B7A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Typing of Listeria monocytogenes by restriction polymorphism of the ribosomal ribonucleic acid gene region
Journal
Zentralbl Bakteriol
ISSN
0934-8840
Publication state
Published
Issued date
02/1992
Peer-reviewed
Oui
Volume
276
Number
3
Pages
356-65
Notes
Journal Article --- Old month value: Feb
Abstract
Ninety four strains of Listeria monocytogenes of different serovars and phagovars as well as of varying origins were characterized by ribosomal RNA gene restriction polymorphism. After digestion by EcoRI or HindIII, chromosomal DNAs were hybridized with a cloned rDNA probe from Bacillus subtilis that included the 16S rRNA gene. The 94 strains were divided into 14 ribovars according to the different hybridization patterns generated by cleavage with EcoRI. Less important genomic heterogeneity could be detected when DNAs were digested by HindIII. EcoRI ribovars analysis allowed to describe a new typing scheme which did not corroborate routine typings such as serotyping and phage typing. It also confirmed a new view of this species in exhibiting a clone gathering most human strains, as first inferred from multilocus enzyme analysis (Piffaretti et al., 22).
Keywords
Animals
Autoradiography
Bacteriophage Typing
DNA Probes
DNA, Bacterial/*analysis
Deoxyribonuclease EcoRI
Deoxyribonuclease HindIII
Humans
Listeria monocytogenes/*classification/genetics
Nucleic Acid Hybridization
*Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
Restriction Mapping
Serotyping
Pubmed
Web of science
Create date
11/02/2008 12:39
Last modification date
20/08/2019 15:30