A novel 90-kDa polypeptide (Tp90) possibly involved in an antigen-independent pathway of T cell activation

Détails

ID Serval
serval:BIB_BCC4C31ADF86
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A novel 90-kDa polypeptide (Tp90) possibly involved in an antigen-independent pathway of T cell activation
Périodique
European Journal of Immunology
Auteur(s)
Carrel  S., Salvi  S., Giuffre  L., Isler  P., Cerottini  J. C.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
06/1987
Volume
17
Numéro
6
Pages
835-41
Notes
Journal Article --- Old month value: Jun
Résumé
A novel surface molecule, Tp90, is described which appears to be involved in an antigen-independent pathway of human T lymphocyte activation. The Tp90 molecule was identified by a monoclonal antibody (mAb), MX20, obtained from a fusion using spleen cells of a mouse immunized with cells from two T cell leukemia lines, Jurkat and HPB-ALL. Biochemical data show that Tp90 is distinct and physically independent from the structures already known to be involved in T cell activation, namely T11, T44 or T3/TCR. These results were confirmed by antibody-induced antigen modulation experiments. Modulation of Tp90 had no effect on the expression of T3 and of the T cell receptor. Conversely, the expression of Tp90 was not affected by modulation of the T3/TCR molecular complex by either anti-T3 or anti-TCR antibody. Functional studies showed that anti-Tp90 mAb MX20 induced high levels of interleukin 2 production in Jurkat cells. Modulation of the T3/TCR complex significantly decreased the response of Jurkat cells to stimulation by antibody MX20, suggesting that the T3/TCR complex regulates the ability of the Tp90 molecule to induce IL 2 synthesis. In addition to its effect on Jurkat cells, anti-Tp90 mAb was found to be mitogenic for peripheral blood T cells. As the magnitude of the proliferative response elicited by anti-Tp90 mAb was lower than that induced by anti-T3 mAb, the possibility was considered that only a subpopulation of T cells is reactive with anti-Tp90. Indeed as determined by FACS analyses, only 3-14% of E-rosette-positive cells were stained with mAb MX20. In addition, multicolor flow cytometry analysis showed that the Tp90+ cells belong preferentially to the CD8 subset.
Mots-clé
Antibodies, Monoclonal/administration & dosage/diagnostic use/immunology Antibody Specificity Antigens, CD27 Antigens, Surface/*analysis/immunology/isolation & purification Binding Sites, Antibody Cell Line Cell Separation Electrophoresis, Polyacrylamide Gel Flow Cytometry Humans Interleukin-2/biosynthesis *Lymphocyte Activation Peptides/isolation & purification Precipitin Tests Radioimmunoassay T-Lymphocytes/classification/*immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
03/03/2018 20:57
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