Growth regulation and co-stimulation of human colorectal cancer cell lines by insulin-like growth factor I, II and transforming growth factor alpha

Details

Serval ID
serval:BIB_B666725B3303
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Growth regulation and co-stimulation of human colorectal cancer cell lines by insulin-like growth factor I, II and transforming growth factor alpha
Journal
British Journal of Cancer
Author(s)
Lahm  H., Suardet  L., Laurent  P. L., Fischer  J. R., Ceyhan  A., Givel  J. C., Odartchenko  N.
ISSN
0007-0920 (Print)
Publication state
Published
Issued date
03/1992
Volume
65
Number
3
Pages
341-6
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
We have tested growth factor responsiveness of a panel of eight human colorectal carcinoma cell lines. Insulin-like growth factors I and II (IGF-I and IGF-II) stimulated growth of five lines (HT-29, LS411N, LS513, SW480, WiDr). At 30 ng ml-1 both factors enhanced growth up to 3-fold. They induced half-maximal stimulation at 1.9-6.51 ng ml-1. Even after delayed addition IGF-I and II significantly enhanced growth in a short-term proliferation assay. They exerted maximal effects under limiting serum conditions (0.5% FCS) and at low cell density (1.25-5 x 10(4) ml-1). Using these conditions transforming growth factor alpha (TGF alpha) enhanced proliferation of all IGF-responsive cell lines, except SW480. 1.11-3.31 ng ml-1 were required to obtain a half-maximal response. With 10-20 ng ml-1 maximal stimulation occurred at plateau values different from those for IGF-I/II. Proliferation of all cell lines responsive to both IGF-I and TGF alpha was further enhanced by combining both factors, resulting a synergistic response of LS513, while the effects on HT-29, LS411N and WiDr were additive. With HT-29 and LS411N a 24 h exposure to TGF alpha was sufficient to obtain a full response in the co-stimulatory assay. Our results illustrate the importance of IGF-I/II and TGF alpha as stimulators of growth of colorectal carcinomas.
Keywords
Cell Division/drug effects Cell Line Colorectal Neoplasms/*pathology Dose-Response Relationship, Drug Drug Synergism Humans Insulin-Like Growth Factor I/*pharmacology Insulin-Like Growth Factor II/*pharmacology Serum Albumin/pharmacology Transforming Growth Factor alpha/*pharmacology
Pubmed
Web of science
Create date
28/01/2008 8:56
Last modification date
20/08/2019 15:24
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