Growth regulation and co-stimulation of human colorectal cancer cell lines by insulin-like growth factor I, II and transforming growth factor alpha
Details
Serval ID
serval:BIB_B666725B3303
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Growth regulation and co-stimulation of human colorectal cancer cell lines by insulin-like growth factor I, II and transforming growth factor alpha
Journal
British Journal of Cancer
ISSN
0007-0920 (Print)
Publication state
Published
Issued date
03/1992
Volume
65
Number
3
Pages
341-6
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
We have tested growth factor responsiveness of a panel of eight human colorectal carcinoma cell lines. Insulin-like growth factors I and II (IGF-I and IGF-II) stimulated growth of five lines (HT-29, LS411N, LS513, SW480, WiDr). At 30 ng ml-1 both factors enhanced growth up to 3-fold. They induced half-maximal stimulation at 1.9-6.51 ng ml-1. Even after delayed addition IGF-I and II significantly enhanced growth in a short-term proliferation assay. They exerted maximal effects under limiting serum conditions (0.5% FCS) and at low cell density (1.25-5 x 10(4) ml-1). Using these conditions transforming growth factor alpha (TGF alpha) enhanced proliferation of all IGF-responsive cell lines, except SW480. 1.11-3.31 ng ml-1 were required to obtain a half-maximal response. With 10-20 ng ml-1 maximal stimulation occurred at plateau values different from those for IGF-I/II. Proliferation of all cell lines responsive to both IGF-I and TGF alpha was further enhanced by combining both factors, resulting a synergistic response of LS513, while the effects on HT-29, LS411N and WiDr were additive. With HT-29 and LS411N a 24 h exposure to TGF alpha was sufficient to obtain a full response in the co-stimulatory assay. Our results illustrate the importance of IGF-I/II and TGF alpha as stimulators of growth of colorectal carcinomas.
Keywords
Cell Division/drug effects
Cell Line
Colorectal Neoplasms/*pathology
Dose-Response Relationship, Drug
Drug Synergism
Humans
Insulin-Like Growth Factor I/*pharmacology
Insulin-Like Growth Factor II/*pharmacology
Serum Albumin/pharmacology
Transforming Growth Factor alpha/*pharmacology
Pubmed
Web of science
Create date
28/01/2008 8:56
Last modification date
20/08/2019 15:24