Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast.

Détails

ID Serval
serval:BIB_B4FB4CCBB9BE
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast.
Périodique
Methods in Cell Biology
Auteur(s)
Ullal P., Bhatia P., Martin S.G.
ISSN
0091-679X (Print)
ISSN-L
0091-679X
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
137
Pages
341-353
Langue
anglais
Résumé
Cytokinesis is an essential cellular event that completes the cell division cycle. It begins with the assembly of an actomyosin contractile ring that undergoes constriction concomitant with the septum formation to divide the cell in two. Placement of the septum at the right position is important to ensure fidelity of the division process. In fission yeast, the medially placed nucleus is a major spatial cue to position the site of division. In this chapter, we describe a simple synthetic biology-based approach to displace the nucleus and study the consequence on division site positioning. We also describe how to perform fluorescence recovery after photobleaching to follow the dynamics of cytokinetic proteins at defined time points by live-cell microscopy.

Mots-clé
Actin Cytoskeleton/ultrastructure, Actomyosin/genetics, Actomyosin/ultrastructure, Cell Nucleus/genetics, Cell Nucleus/ultrastructure, Cytokinesis/genetics, Fluorescence Recovery After Photobleaching/methods, Microscopy/methods, Schizosaccharomyces/genetics, Schizosaccharomyces/ultrastructure, Cell division, Cytokinesis, Division site placement, Fission yeast Schizosaccharomyces pombe, Fluorescence recovery after photobleaching, FRAP, Live-cell microscopy, Nuclear displacement, Synthetic biology
Pubmed
Web of science
Création de la notice
17/01/2017 19:43
Dernière modification de la notice
20/08/2019 16:23
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