Assessment of proliferative activity in breast cancer: MIB-1 immunohistochemistry versus mitotic figure count.

Details

Serval ID
serval:BIB_B4C0F0B802EC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Assessment of proliferative activity in breast cancer: MIB-1 immunohistochemistry versus mitotic figure count.
Journal
Human Pathology
Author(s)
Lehr H.A., Hansen D.A., Kussick S., Li M., Hwang H., Krummenauer F., Trouet S., Gown A.M.
ISSN
0046-8177 (Print)
ISSN-L
0046-8177
Publication state
Published
Issued date
1999
Volume
30
Number
11
Pages
1314-1320
Language
english
Abstract
The proliferative activity is one of the most important single prognostic parameters in breast cancer diagnosis and the time-honored measure of proliferative activity, the mitotic figure count, is an integral component of most combined prognostic scores. The detection of the cell cycle-specific antigens Ki-67, and the development of anti-Ki-67 antibodies, including the paraffin-reactive antibody MIB-1, have established immunohistochemical detection of cell cycle-specific antigens as a measure of proliferative activity in breast cancer diagnosis. The current study was performed to correlate mitotic figure counts with the proliferative activity as assessed by MIB-1 immunohistochemistry, taking into consideration the interobserver reliability of 5 pathologists in estimating mitotic figure counts. In 32 consecutive invasive ductal breast carcinomas, mitotic figure counts were performed independently by 5 pathologists. Mitotic activity was expressed as number of mitotic figures per 10 high-power fields and in a 3-tier score according to the Scarff Bloom Richardson system. Immunohistochemistry was performed using MIB-1 antibody, heat-induced epitope retrieval, and the standard avidin-biotin-immunoperoxidase method. MIB-1 immunohistochemistry was assessed in 3 representative 20x fields by semiquantitative estimation (% of tumor cells positive) and by image analysis (number of MIB-1-positive cells/mm2). We found a high degree of interobserver correlation among 4 experienced pathologists, in both mitotic figures per 10 high-power fields and the 3-tier scoring system. We observed significant, albeit weak, correlations between semiquantitative and quantitative MIB-1 immunohistochemistry and the number of mitotic figures per 10 high-power fields (r between .36 and .53), but this significance was lost in 3 of the 5 observers when mitotic activity was expressed in the 3-tier scoring system. This study confirms mitotic figure counting in the hands of experienced pathologists as a valid, reproducible means of assessing proliferative activity in routine breast cancer diagnosis. The statistically significant, albeit only weak, correlation with MIB-1 immunohistochemistry is in agreement with results obtained by others and suggests that MIB-1 immunohistochemistry cannot be translated by a simple conversion factor into combined prognostic scores to replace the time-honored mitotic figure counts.
Keywords
Antigens, Nuclear, Breast Neoplasms/metabolism, Breast Neoplasms/pathology, Carcinoma, Ductal, Breast/metabolism, Carcinoma, Ductal, Breast/pathology, Cell Division, Humans, Immunohistochemistry, Ki-67 Antigen, Mitotic Index, Nuclear Proteins/metabolism, Observer Variation
Pubmed
Create date
25/11/2011 19:48
Last modification date
20/08/2019 15:23
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