Sand fly salivary proteins induce strong cellular immunity in a natural reservoir of visceral leishmaniasis with adverse consequences for Leishmania.

Détails

ID Serval
serval:BIB_B3685542C501
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Sand fly salivary proteins induce strong cellular immunity in a natural reservoir of visceral leishmaniasis with adverse consequences for Leishmania.
Périodique
PLoS Pathogens
Auteur(s)
Collin N., Gomes R., Teixeira C., Cheng L., Laughinghouse A., Ward J.M., Elnaiem D.E., Fischer L., Valenzuela J.G., Kamhawi S.
ISSN
1553-7374 (Electronic)
ISSN-L
1553-7366
Statut éditorial
Publié
Date de publication
2009
Volume
5
Numéro
5
Pages
e1000441
Langue
anglais
Résumé
Immunity to a sand fly salivary protein protects against visceral leishmaniasis (VL) in hamsters. This protection was associated with the development of cellular immunity in the form of a delayed-type hypersensitivity response and the presence of IFN-gamma at the site of sand fly bites. To date, there are no data available regarding the cellular immune response to sand fly saliva in dogs, the main reservoirs of VL in Latin America, and its role in protection from this fatal disease. Two of 35 salivary proteins from the vector sand fly Lutzomyia longipalpis, identified using a novel approach termed reverse antigen screening, elicited strong cellular immunity in dogs. Immunization with either molecule induced high IgG(2) antibody levels and significant IFN-gamma production following in vitro stimulation of PBMC with salivary gland homogenate (SGH). Upon challenge with uninfected or infected flies, immunized dogs developed a cellular response at the bite site characterized by lymphocytic infiltration and IFN-gamma and IL-12 expression. Additionally, SGH-stimulated lymphocytes from immunized dogs efficiently killed Leishmania infantum chagasi within autologous macrophages. Certain sand fly salivary proteins are potent immunogens obligatorily co-deposited with Leishmania parasites during transmission. Their inclusion in an anti-Leishmania vaccine would exploit anti-saliva immunity following an infective sand fly bite and set the stage for a protective anti-Leishmania immune response.
Mots-clé
Animals, Antibody Formation, Cytokines/genetics, Cytokines/metabolism, Data Interpretation, Statistical, Dogs, Female, Gene Expression, Hypersensitivity, Delayed, Immunity, Cellular, Insect Bites and Stings/immunology, Insect Proteins/genetics, Insect Proteins/immunology, Insect Vectors/genetics, Insect Vectors/immunology, Leishmania infantum/immunology, Leishmania infantum/physiology, Leishmaniasis, Visceral/immunology, Lymphocytes/immunology, Psychodidae/genetics, Psychodidae/immunology, Recombinant Proteins/genetics, Recombinant Proteins/immunology, Salivary Proteins and Peptides/genetics, Salivary Proteins and Peptides/immunology, Skin/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/11/2011 13:07
Dernière modification de la notice
08/05/2019 23:59
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