9-cis-retinoic acid enhances fatty acid-induced expression of the liver fatty acid-binding protein gene.
Details
Serval ID
serval:BIB_B3555CF9CC38
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
9-cis-retinoic acid enhances fatty acid-induced expression of the liver fatty acid-binding protein gene.
Journal
Febs Letters
ISSN
0014-5793[print], 0014-5793[linking]
Publication state
Published
Issued date
1997
Volume
412
Number
3
Pages
480-484
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
The role of retinoic acids (RA) on liver fatty acid-binding protein (L-FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9-cis-Retinoic acid (9-cis-RA) specifically enhanced L-FABP mRNA levels in a time- and dose-dependent manner. The higher induction was found 6 h after addition of 10(-6) M 9-cis-RA in the medium. RA also enhanced further both L-FABP mRNA levels and cytosolic L-FABP protein content induced by oleic acid. The retinoid X receptor (RXR) and the peroxisome proliferator-activated receptor (PPAR), which are known to be activated, respectively, by 9-cis-RA and long chain fatty acid (LCFA), co-operated to bind specifically the peroxisome proliferator-responsive element (PPRE) found upstream of the L-FABP gene. Our result suggest that the PPAR-RXR complex is the molecular target by which 9-cis-RA and LCFA regulate the L-FABP gene.
Keywords
Animals, Carcinoma, Hepatocellular, Carrier Proteins/biosynthesis, Carrier Proteins/genetics, Dimerization, Drug Synergism, Fatty Acid-Binding Proteins, Fatty Acids/pharmacology, Gene Expression Regulation/drug effects, Liver/drug effects, Liver/metabolism, Microbodies/metabolism, Myelin P2 Protein/biosynthesis, Myelin P2 Protein/genetics, Neoplasm Proteins, Nerve Tissue Proteins, RNA, Messenger/drug effects, RNA, Messenger/metabolism, Rats, Receptors, Cytoplasmic and Nuclear/biosynthesis, Receptors, Cytoplasmic and Nuclear/metabolism, Receptors, Retinoic Acid/metabolism, Retinoid X Receptors, Transcription Factors/biosynthesis, Transcription Factors/metabolism, Tretinoin/metabolism, Tretinoin/pharmacology, Tumor Cells, Cultured
Pubmed
Web of science
Create date
24/01/2008 16:04
Last modification date
20/08/2019 15:21
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