Extracellular polysaccharides from Xanthomonas axonopodis pv. manihotis interact with cassava cell walls during pathogenesis

Details

Serval ID
serval:BIB_B0DA294F2588
Type
Article: article from journal or magazin.
Collection
Publications
Title
Extracellular polysaccharides from Xanthomonas axonopodis pv. manihotis interact with cassava cell walls during pathogenesis
Journal
Molecular Plant-Microbe Interactions
Author(s)
Boher  B., Nicole  M., Potin  M., Geiger  J. P.
ISSN
0894-0282 (Print)
Publication state
Published
Issued date
09/1997
Volume
10
Number
7
Pages
803-11
Notes
Journal Article --- Old month value: Sep
Abstract
The location of lipopolysaccharides produced by Xanthomonas axonopodis pv. manihotis during pathogenesis on cassava (Manihot esculenta) was determined by fluorescence and electron microscopy immunolabeling with monoclonal antibodies. During the early stages of infection, pathogen lipopolysaccharides were detected on the outer surface of the bacterial envelope and in areas of the plant middle lamellae in the vicinity of the pathogen. Later in the infection process, lipopolysaccharide-specific antibodies bound to areas where the plant cell wall was heavily degraded. Lipopolysaccharides were not detected in the fibrillar matrix filling intercellular spaces of infected cassava leaves. Monoclonal antibodies specific for the exopolysaccharide xanthan side chain labeled the bacteria, the fibrillar matrix, and portions of the host cell wall. The association of Xanthomonas lipopolysaccharides with host cell walls during plant infection is consistent with a role of these bacterial extracellular polysaccharides in the infection process.
Keywords
Blotting, Western Cell Wall/*metabolism/ultrastructure Cross Reactions Electrophoresis, Polyacrylamide Gel Epitopes/immunology Glucans/metabolism Immunohistochemistry Lipopolysaccharides/*metabolism Manihot/*metabolism/microbiology Microscopy, Electron Microscopy, Fluorescence Pectins/metabolism Xanthomonas/immunology/*metabolism/pathogenicity
Pubmed
Web of science
Create date
24/01/2008 20:10
Last modification date
20/08/2019 15:19
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