Serum-free aggregate cultures of rat CNS glial cells: biochemical, immunocytochemical and morphological characterization.

Details

Serval ID
serval:BIB_AE6909C0C1ED
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Serum-free aggregate cultures of rat CNS glial cells: biochemical, immunocytochemical and morphological characterization.
Journal
Developmental Neuroscience
Author(s)
Guentert-Lauber B., Monnet-Tschudi F., Omlin F.X., Favrod P., Honegger P.
ISSN
0378-5866 (Print)
ISSN-L
0378-5866
Publication state
Published
Issued date
1985
Volume
7
Number
1
Pages
33-44
Language
english
Abstract
Aggregate cultures of mixed glial cells, as well as of enriched astrocytes and oligodendrocytes were prepared, and maintained in serum-free medium for up to 25 days. Biochemical measurements of both neuron-specific and glia-specific enzyme activities showed that these three types of aggregate cultures were virtually devoid of neurons. Astrocyte-enriched cultures were greater than 95% pure, with oligodendrocytes as the only apparent contaminant, whereas oligodendrocyte-enriched cultures still contained a considerable proportion of astrocytes. In all these neuron-free aggregate cultures both astrocytes and oligodendrocytes attained a high degree of maturation. These findings were confirmed by morphological examinations, and by immunofluorescence studies. Furthermore, ultrastructural as well as immunocytochemical investigations using antibodies to myelin basic protein revealed that all three types of glial cell aggregate cultures contained myelin membranes, indicating that the presence of axons is not a prerequisite for the formation of myelin.
Keywords
Animals, Astrocytes/metabolism, Brain, Cell Aggregation, Cell Communication, Cells, Cultured, Microscopy, Electron, Neuroglia/metabolism, Oligodendroglia/metabolism, Rats, Rats, Inbred Strains
Pubmed
Web of science
Create date
24/01/2008 13:12
Last modification date
20/08/2019 15:18
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