The enigma of the gene coding for ribosomal protein S12 in the chloroplasts of Nicotiana.

Détails

ID Serval
serval:BIB_ADBA9A529B1D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The enigma of the gene coding for ribosomal protein S12 in the chloroplasts of Nicotiana.
Périodique
Nucleic Acids Research
Auteur(s)
Fromm H., Edelman M., Koller B., Goloubinoff P., Galun E.
ISSN
0305-1048 (Print)
ISSN-L
0305-1048
Statut éditorial
Publié
Date de publication
1986
Volume
14
Numéro
2
Pages
883-898
Langue
anglais
Résumé
A 2.9 kbp region from within the inverted repeat of Nicotiana chloroplast DNA hybridized with a chloroplast DNA fragment from Euglena containing the complete rps12 gene coding for ribosomal protein S12. Nucleotide sequencing within this region revealed the existance of two rps12 coding stretches interrupted by 540 bp having class II intron structure. Joining and decoding the exon regions produced a sequence of 85 amino acids colinear and 81% homologous to the S12 protein of Euglena chloroplasts and E. coli, starting from amino acid residue 38 to the stop codon. Immediately upstream of codon 38, conserved intron sequences were located. However, the 5' 37 codon of Nicotiana chloroplast rps12 could not be identified by electron microscopy of RNA-DNA hybrids within a DNA region extending 4000 bp upstream of codon 38, nor by computer search of a completely sequenced region extending for more than 9000 bp upstream of this codon. In E. coli, alteration in rps12 codons 42 or 87 causes streptomycin resistance. However, the nucleotide sequence of the identified rps12 exons in two Nicotiana chloroplast mutants resistant to streptomycin were found to be identical to that of wild type.
Mots-clé
Amino Acid Sequence, Base Composition, Base Sequence, Chloroplasts/metabolism, Codon, Genes, Microscopy, Electron, Nucleic Acid Conformation, Plants/genetics, Plants, Toxic, Ribosomal Proteins/genetics, Tobacco/genetics
Pubmed
Création de la notice
24/01/2008 21:02
Dernière modification de la notice
20/08/2019 16:17
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