Article: article from journal or magazin.
A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system.
Journal of Medical Microbiology
Publication types: Journal ArticlePublication Status: ppublish
An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99% for Enterobacteriaceae and 74% for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 and 0.3% for Enterobacteriaceae, and 0.7 and 0.1% for staphylococci, respectively. Thus, bacterial pellets prepared with ammonium chloride allow direct inoculation of VITEK cards with excellent accuracy for Enterobacteriaceae and a lower accuracy for staphylococci.
Anti-Bacterial Agents/pharmacology, Bacteriological Techniques, Drug Resistance, Bacterial, Gram-Negative Bacteria/classification, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/classification, Gram-Positive Bacteria/drug effects, Humans, Microbial Sensitivity Tests, Sensitivity and Specificity
Web of science
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