Determinants of vitellogenin B1 promoter architecture. HNF3 and estrogen responsive transcription within chromatin.

Details

Serval ID
serval:BIB_ABD5B486BDA8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Determinants of vitellogenin B1 promoter architecture. HNF3 and estrogen responsive transcription within chromatin.
Journal
Journal of Biological Chemistry
Author(s)
Robyr D., Gegonne A., Wolffe A.P., Wahli W.
ISSN
0021-9258[print], 0021-9258[linking]
Publication state
Published
Issued date
2000
Volume
275
Number
36
Pages
28291-28300
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The liver-specific vitellogenin B1 promoter is efficiently activated by estrogen within a nucleosomal environment after microinjection into Xenopus laevis oocytes, consistent with the hypothesis that significant nucleosome remodeling over this promoter is not a prerequisite for the activation by the estrogen receptor (ERalpha). This observation lead us to investigate determinants other than ERalpha of chromatin structure and transcriptional activation of the vitellogenin B1 promoter in this system and in vitro. We find that the liver-enriched transcription factor HNF3 has an important organizational role for chromatin structure as demonstrated by DNase I-hypersensitive site mapping. Both HNF3 and the estrogen receptor activate transcription synergistically and are able to interact with chromatin reconstituted in vitro with three positioned nucleosomes. We propose that HNF3 is the cellular determinant which establishes a promoter environment favorable to a rapid transcriptional activation by the estrogen receptor.
Keywords
Animals, Chromatin/drug effects, Chromatin/genetics, DNA Footprinting, DNA-Binding Proteins/metabolism, Deoxyribonuclease I, Estrogen Receptor alpha, Female, Gene Expression Regulation, Hepatocyte Nuclear Factor 3-alpha, Liver/metabolism, Micrococcal Nuclease, Microinjections, Mutagenesis, Site-Directed, Nuclear Proteins/metabolism, Nucleosomes/physiology, Oocytes/physiology, Point Mutation, Promoter Regions, Genetic, Receptors, Estrogen/physiology, Recombinant Proteins/biosynthesis, Transcription Factors/metabolism, Transcription, Genetic/drug effects, Transcription, Genetic/physiology, Vitellogenins/genetics, Vitellogenins/physiology, Xenopus laevis
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 16:04
Last modification date
20/08/2019 15:15
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