Article: article from journal or magazin.
Monoclonal antibodies to arginine vasopressin receptor bind to liver, kidney and pituitary membranes.
Clinical and Experimental Pharmacology and Physiology
Publication types: Journal Article ; Research Support
1. A vasopressin binding protein purified from rat liver membranes was used to immunize Balb/c mice and, subsequently, for the screening of hybrids raised in two different cell fusions. 2. Three hybrids were obtained which secreted monoclonal antibodies (MoAb) that bound to the purified solubilized receptor as detected by an enzyme-linked immunosorbent assay technique. All three MoAb immunoprecipitated the purified receptor. 3. In addition, the MoAb bound in a concentration-dependent manner to crude liver, kidney and anterior pituitary membranes, tissues known to contain arginine vasopressin (AVP) receptors but not to cardiac ventricle membranes which lack AVP receptors. 4. However, the binding of [125I]-[d(CH2)5,Sar7]AVP (a specific radiolabelled V1 antagonist) to the membrane-bound receptor was not inhibited by these antibodies. 5. These results suggest that MoAb recognize epitopes which are common to rat liver, kidney and anterior pituitary membranes but are not at the ligand binding site.
Animals, Antibodies, Monoclonal/immunology, Arginine Vasopressin/analogs & derivatives, Arginine Vasopressin/metabolism, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Kidney/immunology, Kidney/metabolism, Liver/immunology, Liver/metabolism, Mice, Mice, Inbred BALB C, Pituitary Gland/immunology, Pituitary Gland/metabolism, Pituitary Gland, Anterior/immunology, Pituitary Gland, Anterior/metabolism, Rats, Receptors, Vasopressin/immunology, Receptors, Vasopressin/metabolism
Web of science
Last modification date