Monoclonal antibodies to arginine vasopressin receptor bind to liver, kidney and pituitary membranes.

Details

Serval ID
serval:BIB_AA2E8C55DFEE
Type
Article: article from journal or magazin.
Collection
Publications
Title
Monoclonal antibodies to arginine vasopressin receptor bind to liver, kidney and pituitary membranes.
Journal
Clinical and Experimental Pharmacology and Physiology
Author(s)
Trinder D., Mooser V., Phillips P.A., Smith A.I., Casley D., Johnston C.I.
ISSN
0305-1870 (Print)
ISSN-L
0305-1870
Publication state
Published
Issued date
1993
Volume
20
Number
6
Pages
443-449
Language
english
Notes
Publication types: Journal Article ; Research Support
Abstract
1. A vasopressin binding protein purified from rat liver membranes was used to immunize Balb/c mice and, subsequently, for the screening of hybrids raised in two different cell fusions. 2. Three hybrids were obtained which secreted monoclonal antibodies (MoAb) that bound to the purified solubilized receptor as detected by an enzyme-linked immunosorbent assay technique. All three MoAb immunoprecipitated the purified receptor. 3. In addition, the MoAb bound in a concentration-dependent manner to crude liver, kidney and anterior pituitary membranes, tissues known to contain arginine vasopressin (AVP) receptors but not to cardiac ventricle membranes which lack AVP receptors. 4. However, the binding of [125I]-[d(CH2)5,Sar7]AVP (a specific radiolabelled V1 antagonist) to the membrane-bound receptor was not inhibited by these antibodies. 5. These results suggest that MoAb recognize epitopes which are common to rat liver, kidney and anterior pituitary membranes but are not at the ligand binding site.
Keywords
Animals, Antibodies, Monoclonal/immunology, Arginine Vasopressin/analogs & derivatives, Arginine Vasopressin/metabolism, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Kidney/immunology, Kidney/metabolism, Liver/immunology, Liver/metabolism, Mice, Mice, Inbred BALB C, Pituitary Gland/immunology, Pituitary Gland/metabolism, Pituitary Gland, Anterior/immunology, Pituitary Gland, Anterior/metabolism, Rats, Receptors, Vasopressin/immunology, Receptors, Vasopressin/metabolism
Pubmed
Web of science
Create date
13/10/2013 20:59
Last modification date
20/08/2019 15:14
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