Premature birth is associated with not fully differentiated contractile smooth muscle cells in human umbilical artery.

Details

Serval ID
serval:BIB_A80DEF9E8981
Type
Article: article from journal or magazin.
Collection
Publications
Title
Premature birth is associated with not fully differentiated contractile smooth muscle cells in human umbilical artery.
Journal
Placenta
Author(s)
Roffino S., Lamy E., Foucault-Bertaud A., Risso F., Reboul R., Tellier E., Chareyre C., Dignat-George F., Simeoni U., Charpiot P.
ISSN
1532-3102 (Electronic)
ISSN-L
0143-4004
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
33
Number
6
Pages
511-517
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Smooth muscle cells (SMCs) participate to the regulation of peripheral arterial resistance and blood pressure. To assume their function, SMCs differentiate throughout the normal vascular development from a synthetic phenotype towards a fully differentiated contractile phenotype by acquiring a repertoire of proteins involved in contraction. In human fetal muscular arteries and umbilical arteries (UAs), no data are available regarding the differentiation of SMCs during the last trimester of gestation. The objective of this study was to characterize the phenotype of SMCs during this gestation period in human UAs. We investigated the phenotype of SMCs in human UAs from very preterm (28-31 weeks of gestation), late preterm (32-35 weeks) and term (37-41 weeks) newborns using biochemical and immunohistochemical detection of α-actin, smooth muscle myosin heavy chain, smoothelin, and non-muscle myosin heavy chain. We found that the number of SMCs positive for smoothelin in UAs increased with gestational age. Western blot analysis revealed a higher content of smoothelin in term compared to very preterm UAs. These results show that SMCs in human UAs gradually acquire a fully differentiated contractile phenotype during the last trimester of gestation and thus that premature birth is associated with not fully differentiated contractile SMCs in human UAs.
Keywords
Actins/metabolism, Cell Differentiation, Cytoskeletal Proteins/biosynthesis, Female, Humans, Infant, Newborn, Infant, Premature, Muscle Proteins/biosynthesis, Muscle, Smooth, Vascular/cytology, Myosin Heavy Chains/biosynthesis, Pregnancy, Pregnancy Trimester, Third, Premature Birth, Umbilical Arteries/cytology
Pubmed
Create date
22/02/2015 10:43
Last modification date
20/08/2019 15:12
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