Torsional stress induces left-handed helical stretches in DNA of natural base sequence: circular dichroism and antibody binding.

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Serval ID
serval:BIB_A6C0ABEDA135
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Production externe
Title
Torsional stress induces left-handed helical stretches in DNA of natural base sequence: circular dichroism and antibody binding.
Journal
EMBO Journal
Author(s)
Di Capua E., Stasiak A., Koller T., Brahms S., Thomae R., Pohl F.M.
ISSN
0261-4189[print], 0261-4189[linking]
Publication state
Published
Issued date
1983
Peer-reviewed
Oui
Volume
2
Number
9
Pages
1531-1535
Language
english
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Above a threshold of torsional stress, the c.d. spectrum of covalently closed circular DNA of natural base sequence acquires a Z-like contribution and antibodies raised against Z-DNA are bound. Mapping of the antibody binding sites by electron microscopy reveals sites which correlate with stretches enriched in alternating purine-pyrimidine sequences and GC base pairs.
Keywords
Animals, Antibodies, Base Sequence, Binding Sites, Circular Dichroism, DNA, Circular/chemistry, DNA, Circular/genetics, Globins/genetics, Mice, Microscopy, Electron, Nucleic Acid Conformation, Plasmids/chemistry, Plasmids/genetics, Rabbits, Stress, Mechanical
Pubmed
11892807
Web of science
A1983RE72300016
Create date
24/01/2008 11:36
Last modification date
20/08/2019 16:11
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