Production and characterization of a rabbit antiserum to the mouse CD8 antigenic complex by immunization with a synthetic peptide

Details

Serval ID
serval:BIB_A37EFDFE224A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Production and characterization of a rabbit antiserum to the mouse CD8 antigenic complex by immunization with a synthetic peptide
Journal
Journal of Immunological Methods
Author(s)
Brunati  S., Corradin  G., Bron  C.
ISSN
0022-1759 (Print)
Publication state
Published
Issued date
01/1987
Volume
96
Number
1
Pages
97-105
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 26
Abstract
A rabbit antiserum to the mouse CD8 antigen (Lyt-2/3) was obtained through the use of a synthetic peptide corresponding to the N-terminal segment of the 37 kDa subunit of the CD8 molecular complex. This anti-peptide antiserum detected specifically the membrane antigen from detergent extracts of surface-labeled mouse thymocytes. The efficiency of the immunoprecipitation increased significantly upon treatment of the cell lysates with 0.1% SDS at 100 degrees C before immunopurification. Finally both the 37 kDa and the 32 kDa polypeptides expressing the Lyt-2 antigen were revealed by immunoblotting.
Keywords
Animals Antibodies, Monoclonal/immunology Antibody Specificity Antigens, Differentiation, T-Lymphocyte Antigens, Surface/*immunology *Immune Sera Immunization Immunoelectrophoresis Membrane Proteins/immunology Mice Peptide Fragments/chemical synthesis/*immunology Rabbits T-Lymphocytes/immunology
Pubmed
Web of science
Create date
24/01/2008 14:55
Last modification date
20/08/2019 15:09
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