Preeclamptic plasma induces transcription modifications involving the AP-1 transcriptional regulator JDP2 in endothelial cells.

Details

Serval ID
serval:BIB_A236AC9C86FC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Preeclamptic plasma induces transcription modifications involving the AP-1 transcriptional regulator JDP2 in endothelial cells.
Journal
American Journal of Pathology
Author(s)
Calicchio R., Buffat C., Mathieu J.R., Ben Salem N., Mehats C., Jacques S., Hertig A., Berkane N., Grevoul-Fresquet J., Simeoni U., Peyssonnaux C., Gavard J., Vaiman D., Miralles F.
ISSN
1525-2191 (Electronic)
ISSN-L
0002-9440
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
183
Number
6
Pages
1993-2006
Language
english
Notes
Publication types: Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
Preeclampsia is a pregnancy disorder characterized by hypertension and proteinuria. In preeclampsia, the placenta releases factors into the maternal circulation that cause a systemic endothelial dysfunction. Herein, we investigated the effects of plasma from women with preeclamptic and normal pregnancies on the transcriptome of an immortalized human umbilical vein endothelial cell line. The cells were exposed for 24 hours to preeclamptic or normal pregnancy plasma and their transcriptome was analyzed using Agilent microarrays. A total of 116 genes were found differentially expressed: 71 were up-regulated and 45 were down-regulated. In silico analysis revealed significant consistency and identified four functional categories of genes: mitosis and cell cycle progression, anti-apoptotic, fatty acid biosynthesis, and endoplasmic reticulum stress effectors. Moreover, several genes involved in vasoregulation and endothelial homeostasis showed modified expression, including EDN1, APLN, NOX4, and CBS. Promoter analysis detected, among the up-regulated genes, a significant overrepresentation of genes containing activation protein-1 regulatory sites. This correlated with down-regulation of JDP2, a gene encoding a repressor of activation protein-1. The role of JDP2 in the regulation of a subset of genes in the human umbilical vein endothelial cells was confirmed by siRNA inhibition. We characterized transcriptional changes induced by preeclamptic plasma on human umbilical vein endothelial cells, and identified, for the first time to our knowledge, JDP2 as a regulator of a subset of genes modified by preeclamptic plasma.
Keywords
Adult, Cell Line, Transformed, Endothelial Cells/metabolism, Endothelial Cells/pathology, Female, Gene Expression Regulation, Humans, Plasma/metabolism, Pre-Eclampsia/blood, Pre-Eclampsia/pathology, Pregnancy, Repressor Proteins/metabolism, Transcription Factor AP-1/metabolism, Transcriptome
Pubmed
Web of science
Create date
22/02/2015 9:03
Last modification date
20/08/2019 15:08
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