Enhanced expression of HIV antigens and improved antigen presentation after infection with replication competent attenuated vaccinia virus in vitro : P17-23

Détails

ID Serval
serval:BIB_9FF37A338ADA
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Titre
Enhanced expression of HIV antigens and improved antigen presentation after infection with replication competent attenuated vaccinia virus in vitro : P17-23
Titre de la conférence
AIDS Vaccine 2009
Auteur(s)
Quakkelaar E., Redeker A., Loof N., Perdiguero B., Heinen P., Nieto J., Esteban M., Kibler K., Jacobs B., Pantaleo G., Melief C.
Adresse
Paris, France, October 19-22, 2009
ISBN
1742-4690
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
6
Série
Retrovirology
Pages
305
Langue
anglais
Notes
Background : Poxvirus vectors, particularly the attenuated and host-range restricted ALVAC, MVA and NYVAC, have been extensively used to develop the so-called T-cell vaccines. ALVAC has been a major component of a prime/boost vaccine regimen currently tested in phase III clinical trial and MVA and NYVAC have shown good levels of immunogenicity in combination with DNA priming. Despite the promising immunogenicity data, there is common agreement that the poxvirus vector T-cell vaccines need to be improved.
Methods : In order to improve the immunogenicity of NYVAC we have used two strategies: a) development of attenuated replication competent (in human cells) NYVAC and b) deletion of poxvirus genes known to encode for proteins that may affect the immune response. The newly generated replication competent or gene deletion NYVAC mutants expressing env, gag, pol and nef have been evaluated for their ability to stimulate HIV-specific CD8 T-cell responses from blood mononuclear cells of HIV-infected subjects. HIV-specific immune responses induced by the new NYVAC vectors were evaluated in newly developed direct and cross-presentation assays in vitro and compared with the parental replication deficient NYVAC vector. Finally, expression of gag following infection of different target cells (dendritic cells and HeLa cells) with replication competent and deficient NYVAC vectors was also evaluated.
Results : The results obtained indicated that replication competent NYVAC vectors induce: a) improvement in direct and cross-presentation, and b) substantial increase in the expression of gag in the infected target cells.
Conclusion : This project is funded under the CAVD program.
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Création de la notice
21/12/2009 12:50
Dernière modification de la notice
03/03/2018 20:04
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