Molecular identification of Fusarium species in onychomycoses

Détails

ID Serval
serval:BIB_9FE3B59453A5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular identification of Fusarium species in onychomycoses
Périodique
Dermatology
Auteur(s)
Ninet  B., Jan  I., Bontems  O., Lechenne  B., Jousson  O., Lew  D., Schrenzel  J., Panizzon  R. G., Monod  M.
ISSN
1018-8665 (Print)
Statut éditorial
Publié
Date de publication
2005
Volume
210
Numéro
1
Pages
21-5
Notes
Journal Article
Research Support, Non-U.S. Gov't
Résumé
BACKGROUND: Fusarium species are isolated from about 3% of onychomycoses in the Swiss native population. On the basis of macroscopic characters and microscopic examination of the cultures, identification of Fusarium often remains difficult or uncertain because of variations from one isolate to another and overlapping characteristics between species. OBJECTIVE: To obtain information about the prevailing species of Fusarium collected from onychomycoses. METHODS: An analysis of the Fusarium specimens isolated in the Department of Dermatology at the University Hospital of Lausanne was conducted during a 2-year period (71 isolates). A 311-bp fragment of the gene encoding 28S rDNA was amplified by PCR and sequenced. DNA sequences were compared to those available for reference strains. RESULTS: Fusarium oxysporum was the most frequently isolated species, accounting for 54% of the isolates. F. proliferatum and 4 taxons belonging to the F. solani species complex were identified with an appreciable frequency ranging from 4 to 14%. CONCLUSION: The Fusarium species identified were the same as those known to cause disseminated fusariosis in immunocompromised patients. The presence of these Fusarium species in onychomycoses warrants that careful attention should be paid to abnormal nails before beginning immunosuppressive treatments in patients.
Mots-clé
Base Sequence DNA Primers DNA, Bacterial/analysis DNA, Ribosomal/analysis Fusarium/classification/genetics/*isolation & purification Humans Molecular Sequence Data Onychomycosis/*epidemiology/*microbiology Polymerase Chain Reaction Prevalence Switzerland/epidemiology
Pubmed
Web of science
Création de la notice
25/01/2008 16:55
Dernière modification de la notice
20/08/2019 15:06
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