Effects of polymer-coated multi-wall carbon nanotubes on mouse RAW 264.7 macrophages

Détails

ID Serval
serval:BIB_9F18349E8C86
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Titre
Effects of polymer-coated multi-wall carbon nanotubes on mouse RAW 264.7 macrophages
Titre de la conférence
American Thoracic Society 2009 International Conference, May 15-20, 2009, San Diego, California
Auteur(s)
Tabet Lyes, Bussy Cyrill, Setyan Ari, Boyer Laurent, Desqueyroux Hélène, Rossi Michel J., Boczkowski Jorge, Lanone Sophie
ISBN
1073-449X
Statut éditorial
Publié
Date de publication
2009
Volume
179
Série
American Journal of Respiratory and Critical Care Medicine
Pages
A5250
Langue
anglais
Notes
SAPHIRID:80974
Résumé
Surface characteristics (area, chemical reactivity) play an important role in cell response to nanomaterials. The aim of this study was to evaluate the oxidative and inflammatory effects of multi−wall carbon nanotubes (MWCNT) uncoated (P0) or coated with carboxylic polyacid or polystyrene polybutadiene polymetacrylate of methyl polymers (P1 and P2 respectively) on murine macrophages (RAW 264.7 cell line). Carbon black nanoparticles (CB, diameter 95 nm) and crocidolite fibers (diameter: 80 nm, length: < 10 μm) were used as controls. Surface functional groups present on MWCNTs were analyzed by Knudsen flow reactor. The amount of acidic sites was P1> P0> P2, for basic sites was P0> P1>> P2 and for oxidizable sites was P0> P2> P1. In contact with cells, P2 formed smaller aggregates than P0 and P1, which were of similar size. Optical microscopy showed the formation of vacuoles after exposure only to P0, P1 and crocidolite. Incubation of cells with P0, P1 and crocidolite fibers induced a significant and similar decrease in metabolic activity, whereas P2 and CB had no effect. Cell number and membrane permeability were unmodified by incubation with the different particles. Incubation of macrophages with P0, P1 and crocidolite induced a dose− and time−dependent increase in mRNA expression of oxidative stress marker (HO−1, GPX1) and inflammatory mediators (TNF−a, MIP−2). No such responses were observed with P2 and CB. In conclusion, MWCNT coated with a carboxylic polyacid polymer exerted similar oxidative and inflammatory effects to uncoated MWCNT. By contrast, no such effects were observed with MWCNT coated with a polystyrene−based polymer. This kind of coating could be useful to decrease MWCNT toxicity.
Mots-clé
Nanotubes, Carbon , Macrophages , Nanoparticles , Asbestos, Crocidolite , Oxidative Stress
Création de la notice
27/01/2010 13:20
Dernière modification de la notice
03/03/2018 20:02
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