The small RNA PhrS stimulates synthesis of the Pseudomonas aeruginosa quinolone signal.

Details

Serval ID
serval:BIB_9ED9F56F63CD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The small RNA PhrS stimulates synthesis of the Pseudomonas aeruginosa quinolone signal.
Journal
Molecular Microbiology
Author(s)
Sonnleitner E., Gonzalez N., Sorger-Domenigg T., Heeb S., Richter A.S., Backofen R., Williams P., Hüttenhofer A., Haas D., Bläsi U.
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Publication state
Published
Issued date
2011
Volume
80
Number
4
Pages
868-885
Language
english
Abstract
Quorum sensing, a cell-to-cell communication system based on small signal molecules, is employed by the human pathogen Pseudomonas aeruginosa to regulate virulence and biofilm development. Moreover, regulation by small trans-encoded RNAs has become a focal issue in studies of virulence gene expression of bacterial pathogens. In this study, we have identified the small RNA PhrS as an activator of PqsR synthesis, one of the key quorum-sensing regulators in P. aeruginosa. Genetic studies revealed a novel mode of regulation by a sRNA, whereby PhrS uses a base-pairing mechanism to activate a short upstream open reading frame to which the pqsR gene is translationally coupled. Expression of phrS requires the oxygen-responsive regulator ANR. Thus, PhrS is the first bacterial sRNA that provides a regulatory link between oxygen availability and quorum sensing, which may impact on oxygen-limited growth in P. aeruginosa biofilms.
Keywords
Bacterial Proteins/metabolism, Base Sequence, Blotting, Northern, Gene Expression Regulation, Bacterial, Microarray Analysis, Open Reading Frames/genetics, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism, Quinolones/metabolism, Quorum Sensing, RNA, Bacterial/genetics, Sequence Analysis, RNA, Signal Transduction, Trans-Activators/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
16/02/2012 9:45
Last modification date
20/08/2019 15:05
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