The lactotripeptides isoleucine-proline-proline and valine-proline-proline do not inhibit the N-terminal or C-terminal angiotensin converting enzyme active sites in humans.

Details

Serval ID
serval:BIB_9ED6A8624DFC
Type
Article: article from journal or magazin.
Collection
Publications
Title
The lactotripeptides isoleucine-proline-proline and valine-proline-proline do not inhibit the N-terminal or C-terminal angiotensin converting enzyme active sites in humans.
Journal
Journal of Hypertension
Author(s)
Wuerzner G., Peyrard S., Blanchard A., Lalanne F., Azizi M.
ISSN
1473-5598 (Electronic)
ISSN-L
0263-6352
Publication state
Published
Issued date
2009
Volume
27
Number
7
Pages
1404-1409
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
BACKGROUND: The potential blood pressure lowering effect of fermented milk may involve inhibition of angiotensin-converting enzyme (ACE) by dairy lactotripeptides generated during milk fermentation, such as isoleucine-proline-proline (IPP) and valine-proline-proline (VPP). These peptides are weak ACE inhibitors in vitro but it remains unclear whether they inhibit ACE in vivo in humans.
OBJECTIVE: To assess in vivo ACE inhibition in individuals given fermented milk over a 7-day period.
DESIGN AND METHODS: Twelve healthy normotensive men were given 330 ml of fermented milk once daily from day 1 to day 7 (IPP: 4.5 mg and VPP: 6.6 mg) and a single dose of 50 mg captopril on day 8. ACE inhibition was assessed in vivo by measuring plasma and urine AcSDKP and plasma active renin and in vitro by measuring plasma ACE activity using hippuryl-histidine-leucine. Plasma IPP/VPP concentrations were measured by LC/MS/MS.
RESULTS: Plasma IPP concentrations increased slightly and very transiently after fermented milk administration. Plasma VPP concentrations were below the limit of quantification. Fermented milk had no effect on plasma AcSDKP, ACE activity or active renin concentrations on days 1 or 7. Urine AcSDKP excretion underwent a small transient increase. In contrast, plasma and urine AcSDKP increased 7.7-fold and 70-fold, respectively, and plasma ACE activity decreased by 82.3 +/- 16.1% following captopril administration; plasma active renin concentration increased four-fold.
CONCLUSION: IPP and VPP were poorly absorbed and rapidly eliminated. They did not inhibit plasma or endothelial ACE in vivo at the selected doses and had no specific effect on the N-terminal or C-terminal ACE domains.
Keywords
Animals, Catalytic Domain, Fermentation, Humans, Male, Milk, Oligopeptides/blood, Oligopeptides/pharmacology, Peptidyl-Dipeptidase A/drug effects, Peptidyl-Dipeptidase A/metabolism, Reference Values, Renin/blood
Pubmed
Web of science
Create date
13/03/2013 15:35
Last modification date
20/08/2019 15:05
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