Controlled expression of recombinant proteins in Physcomitrella patens by a conditional heat-shock promoter: a tool for plant research and biotechnology.

Détails

Ressource 1Télécharger: serval:BIB_9E1288D32DF8.P001 (493.39 [Ko])
Etat: Public
Version: de l'auteur
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
ID Serval
serval:BIB_9E1288D32DF8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Controlled expression of recombinant proteins in Physcomitrella patens by a conditional heat-shock promoter: a tool for plant research and biotechnology.
Périodique
Plant Molecular Biology
Auteur(s)
Saidi Y., Finka A., Chakhporanian M., Zrÿd J.P., Schaefer D.G., Goloubinoff P.
ISSN
0167-4412 (Print)
ISSN-L
0167-4412
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
59
Numéro
5
Pages
697-711
Langue
anglais
Résumé
The ability to express tightly controlled amounts of endogenous and recombinant proteins in plant cells is an essential tool for research and biotechnology. Here, the inducibility of the soybean heat-shock Gmhsp17.3B promoter was addressed in the moss Physcomitrella patens, using beta-glucuronidase (GUS) and an F-actin marker (GFP-talin) as reporter proteins. In stably transformed moss lines, Gmhsp17.3B-driven GUS expression was extremely low at 25 degrees C. In contrast, a short non-damaging heat-treatment at 38 degrees C rapidly induced reporter expression over three orders of magnitude, enabling GUS accumulation and the labelling of F-actin cytoskeleton in all cell types and tissues. Induction levels were tightly proportional to the temperature and duration of the heat treatment, allowing fine-tuning of protein expression. Repeated heating/cooling cycles led to the massive GUS accumulation, up to 2.3% of the total soluble proteins. The anti-inflammatory drug acetyl salicylic acid (ASA) and the membrane-fluidiser benzyl alcohol (BA) also induced GUS expression at 25 degrees C, allowing the production of recombinant proteins without heat-treatment. The Gmhsp17.3B promoter thus provides a reliable versatile conditional promoter for the controlled expression of recombinant proteins in the moss P. patens.
Mots-clé
Aspirin/pharmacology, Benzyl Alcohol/pharmacology, Biotechnology/methods, Bryopsida/drug effects, Bryopsida/genetics, Dose-Response Relationship, Drug, Enzyme Induction, Gene Expression Regulation, Enzymologic/genetics, Gene Expression Regulation, Plant/drug effects, Gene Expression Regulation, Plant/genetics, Heat-Shock Proteins/genetics, Plant Proteins/genetics, Plant Proteins/metabolism, Promoter Regions, Genetic/genetics, Recombinant Proteins/genetics, Recombinant Proteins/metabolism, Temperature, Time
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 20:02
Dernière modification de la notice
01/10/2019 6:19
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