A comparison of cold storage solutions for pancreas preservation prior to islet isolation
Details
Serval ID
serval:BIB_9BFFC13C0C17
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A comparison of cold storage solutions for pancreas preservation prior to islet isolation
Journal
Transplant Proc
ISSN
0041-1345 (Print)
ISSN-L
0041-1345
Publication state
Published
Issued date
10/2005
Volume
37
Number
8
Pages
3396-7
Language
english
Notes
Wojtusciszyn, A
Bosco, D
Morel, Ph
Baertschiger, R
Armanet, M
Kempf, M C
Badet, L
Toso, C
Berney, T
eng
Comparative Study
Transplant Proc. 2005 Oct;37(8):3396-7. doi: 10.1016/j.transproceed.2005.09.019.
Bosco, D
Morel, Ph
Baertschiger, R
Armanet, M
Kempf, M C
Badet, L
Toso, C
Berney, T
eng
Comparative Study
Transplant Proc. 2005 Oct;37(8):3396-7. doi: 10.1016/j.transproceed.2005.09.019.
Abstract
BACKGROUND: Several solutions are used to preserve the pancreas prior to islet isolation. This study sought to assess whether the type of solution had an impact on the isolation outcome. METHODS: We reviewed data from 125 islet isolation procedures performed from January 2002 to January 2005. Pancreata were preserved in University of Wisconsin (UW) (n = 101), Celsior (CS) (n = 19), or IGL-1 (n = 5) solutions. Islet isolation results and transplantation rates were compared between groups. RESULTS: UW, CS, and IGL-1 groups were similar according to donor's age, weight, and body mass index. Weight of undigested pancreas was 20 +/- 13.1, 21.4 +/- 15.7, and 17.4 +/- 8.7 g for UW, CS, and IGL-1, respectively (P > .2). Final total number of IEQ was 267,000 +/- 132,000, 277,000 +/- 155,000, and 311,000 +/- 163,000, respectively (P > .4). Success rate (defined as >250,000 IEQ) was 55.5%, 52.9%, and 60% for UW, Celsior, and IGL-1 (P > .9); the transplantation rate was 42.2% for UW, 36.8% for Celsior, and 80% for IGL-1 preservation (P > .2). CONCLUSIONS: In this preliminary study, UW, Celsior, and IGL-1 solutions demonstrated similar islet isolation results. The new IGL-1 solution appears promising.
Keywords
Adenosine, Allopurinol, Disaccharides, Electrolytes, Glutamates, Glutathione, Histidine, Humans, Insulin, Islets of Langerhans/*cytology, Islets of Langerhans Transplantation, Mannitol, *Organ Preservation Solutions, *Pancreas, Raffinose, Tissue and Organ Harvesting/*methods
Pubmed
Create date
14/06/2021 9:59
Last modification date
18/09/2021 6:38