Integrase-Mediated Recombination of the bel-1 Gene Cassette Encoding the Extended-Spectrum β-Lactamase BEL-1.

Details

Serval ID
serval:BIB_9B5853587B79
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Integrase-Mediated Recombination of the bel-1 Gene Cassette Encoding the Extended-Spectrum β-Lactamase BEL-1.
Journal
Antimicrobial agents and chemotherapy
Author(s)
Bouheraoua N., Poirel L., Bourreau B., Bonnin R., Laroche L., Naas T., Nordmann P.
ISSN
1098-6596 (Electronic)
ISSN-L
0066-4804
Publication state
Published
Issued date
05/2018
Peer-reviewed
Oui
Volume
62
Number
5
Pages
e00030-18
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Integrons are genetic elements that can acquire and rearrange gene cassettes. The bla <sub>BEL-1</sub> gene encodes an extended-spectrum β-lactamase, BEL-1, that is present at the second position of the variable region of class 1 integrons identified in Pseudomonas aeruginosa The mobility of the bel-1 gene cassette was analyzed under physiological conditions and with the integrase gene being overexpressed. Cassette mobility in Escherichia coli was detected by excision/integration into the recipient integron In3 on the conjugative plasmid R388 with the overproduced integrase. Despite several antibiotic pressures, the bel-1 cassette remained at the second position in the integron, highlighting its stability in P. aeruginosa Overexpression of the integrase gene in E. coli induced bel-1 cassette recombination. However, cassettes containing two genes (bla <sub>BEL-1</sub> and smr2 or bla <sub>BEL-1</sub> and aacA4) were excised, suggesting that the bel-1 cassette attC site was defective. We show that bel-1 is a stable gene cassette under physiological growth conditions, irrespective of the selective antibiotic pressure, that may be mobilized upon overexpression of the integrase gene.
Keywords
Anti-Bacterial Agents/pharmacology, DNA, Bacterial/genetics, Escherichia coli/drug effects, Escherichia coli/enzymology, Escherichia coli/genetics, Integrases/genetics, Integrons/genetics, Pseudomonas aeruginosa/drug effects, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics, Recombination, Genetic/genetics, beta-Lactamases/genetics, BEL-1, ESBL, Pseudomonas aeruginosa, integron, site-specific recombination
Pubmed
Web of science
Open Access
Yes
Create date
03/03/2018 14:13
Last modification date
20/08/2019 15:02
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