Characterization of nonstructural protein membrane anchor deletion mutants expressed in the context of the hepatitis C virus polyprotein
Details
Serval ID
serval:BIB_9B2D0C4CB43A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization of nonstructural protein membrane anchor deletion mutants expressed in the context of the hepatitis C virus polyprotein
Journal
Journal of Virology
ISSN
0022-538X (Print)
Publication state
Published
Issued date
06/2005
Volume
79
Number
12
Pages
7911-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Protein-protein interactions involved in formation of the membrane-associated hepatitis C virus (HCV) replication complex are poorly understood. Here, we investigated nonstructural proteins with deletions in their membrane anchor domains when expressed in the context of the entire HCV polyprotein. Interactions among cytosolic domains of HCV nonstructural proteins were found not to be sufficiently strong to rescue such mutants to the membrane. Thus, the membrane anchor domains of nonstructural proteins are essential for incorporation of these proteins into the HCV replication complex while interactions among the cytosolic domains appear to be relatively weak. This feature may provide the nonstructural proteins with a certain flexibility to perform their multiple functions during HCV replication.
Keywords
Cell Line, Tumor
Cell Membrane/metabolism
*Gene Deletion
Hepacivirus/genetics/metabolism/*physiology
Humans
Membrane Microdomains
Mutation
Polyproteins/*metabolism
Subcellular Fractions/metabolism
Viral Nonstructural Proteins/genetics/*metabolism
Virus Replication
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:05
Last modification date
20/08/2019 15:02