A DNA-Modified Live Vaccine Prime-Boost Strategy Broadens the T-Cell Response and Enhances the Antibody Response against the Porcine Reproductive and Respiratory Syndrome Virus.

Détails

ID Serval
serval:BIB_958F299A4F67
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A DNA-Modified Live Vaccine Prime-Boost Strategy Broadens the T-Cell Response and Enhances the Antibody Response against the Porcine Reproductive and Respiratory Syndrome Virus.
Périodique
Viruses
Auteur(s)
Bernelin-Cottet C., Urien C., Stubsrud E., Jakob V., Bouguyon E., Bordet E., Barc C., Boulesteix O., Contreras V., Barnier-Quer C., Collin N., Trus I., Nauwynck H., Bertho N., Schwartz-Cornil I.
ISSN
1999-4915 (Electronic)
ISSN-L
1999-4915
Statut éditorial
Publié
Date de publication
14/06/2019
Peer-reviewed
Oui
Volume
11
Numéro
6
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
The Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) induces reproductive disorders in sows and respiratory illnesses in growing pigs and is considered as one of the main pathogenic agents responsible for economic losses in the porcine industry worldwide. Modified live PRRSV vaccines (MLVs) are very effective vaccine types against homologous strains but they present only partial protection against heterologous viral variants. With the goal to induce broad and cross-protective immunity, we generated DNA vaccines encoding B and T antigens derived from a European subtype 1 strain that include T-cell epitope sequences known to be conserved across strains. These antigens were expressed either in a native form or in the form of vaccibodies targeted to the endocytic receptor XCR1 and CD11c expressed by different types of antigen-presenting cells (APCs). When delivered in skin with cationic nanoparticles and surface electroporation, multiple DNA vaccinations as a stand-alone regimen induced substantial antibody and T-cell responses, which were not promoted by targeting antigens to APCs. Interestingly, a DNA-MLV prime-boost strategy strongly enhanced the antibody response and broadened the T-cell responses over the one induced by MLV or DNA-only. The anti-nucleoprotein antibody response induced by the DNA-MLV prime-boost was clearly promoted by targeting the antigen to CD11c and XCR1, indicating a benefit of APC-targeting on the B-cell response. In conclusion, a DNA-MLV prime-boost strategy, by enhancing the potency and breadth of MLV vaccines, stands as a promising vaccine strategy to improve the control of PRRSV in infected herds.
Mots-clé
DNA vaccine, PRRSV, antigen-presenting cell targeting, modified-live vaccine, pigs
Pubmed
Open Access
Oui
Création de la notice
24/06/2019 17:50
Dernière modification de la notice
21/08/2019 6:36
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