Murine leukemia virus proteins expressed on the surface of infected cells in culture

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serval:BIB_93CF042BA029
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Murine leukemia virus proteins expressed on the surface of infected cells in culture
Journal
Journal of Virology
Author(s)
Buetti E., Diggelmann H.
ISSN
0022-538X (Print)
Publication state
Published
Issued date
03/1980
Volume
33
Number
3
Pages
936-44
Notes
Journal Article --- Old month value: Mar
Abstract
Infection of JLS-V9 cells in culture with Rauscher murine leukemia virus induced the appearance on the cell surface of two classes of viral proteins: Rauscher murine leukemia virus gp70, and glycoproteins related to the viral core (gag) proteins with apparent molecular weights in sodium dodecyl sulfate polyacrylamide gels of 80 x 10(3) and 95 x 10(3). The latter proteins were identified by lactoperoxidase-catalyzed iodination of the cell surface and by metabolic labeling with [(3)H]mannose followed by immunoprecipitation with an antiserum directed against the major viral core protein, p30. Tryptic peptide maps of chloramine T-iodinated proteins indicated that 80 x 10(3) - and 95 x 10(3)-molecular-weight proteins were closely related. The 95 x 10(3)-molecular-weight protein from Rauscher murine leukemia virus-infected cells had a tyrosine fingerprint which was identical to that of the 95 x 10(3)-molecular-weight gag surface polyprotein of endogenous virus-producing AKR-A cells, suggesting that expression on the cell surface of glycosylated forms of gag precursor polyproteins may not be an exclusive property of leukemic thymocytes, but a more general phenomenon in murine leukemia virus infection. Tryptic fingerprint analysis of iodinated viral and cell-bound gp70's before and after desialylation indicated a lower level of glycosylation in the cell-bound gp70 population than in virions. Analysis of only surface-iodinated gp70 showed a simple pattern of exposed tryptic peptides which was very similar in Rauscher murine leukemia virus-infected cells and in AKR-A cells.
Keywords
Animals Bone Marrow Cell Line Cell Membrane/*analysis Glycoproteins/*analysis Membrane Proteins/*analysis Mice Molecular Weight Peptides/analysis Rauscher Virus/growth & development/*metabolism Viral Proteins/*analysis
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25/01/2008 14:25
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20/08/2019 14:56
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