Comparison of five poxvirus genomes by analysis with restriction endonucleases HindIII, BamI and EcoRI.

Détails

ID Serval
serval:BIB_93C4F6517D33
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparison of five poxvirus genomes by analysis with restriction endonucleases HindIII, BamI and EcoRI.
Périodique
Journal of General Virology
Auteur(s)
Müller H.K., Wittek R., Schaffner W., Schümperli D., Menna A., Wyler R.
ISSN
0022-1317[print], 0022-1317[linking]
Statut éditorial
Publié
Date de publication
01/1978
Volume
38
Numéro
1
Pages
135-147
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The DNAs of rabbitpox, vaccinia, Cowpox, ectromelia and fowl pox virus, all
grown in the same host system, were cleaved with restriction endonucleases
HindIII, BarnI and EcoRI. The resulting digestion products were separated by
electrophoresis on agarose slab gels. From the similarities and dissimilarities of
the migration profiles obtained, it was concluded that vaccinia and rabbitpox virus
are genetically the most closely related viruses investigated, although their DNA
cleavage patterns are clearly distinguishable. Cowpox and ectromelia virus both
show about the same degree of relatedness to each other as to rabbitpox and
vaccinia virus. Fowl pox virus, which belongs to the genus Avipoxvirus, contains
the largest genome examined (approx. I6O × IO
6 daltons) and the cleavage patterns
of its DNA show no similarities with those of orthopoxvirus DNAs, thus indicat-
ing a very low degree of genetic relatedness. We believe that restriction analysis is
a useful method for the identification and classification of poxvirus isolates.
Mots-clé
Animals, Chick Embryo, Culture Techniques, DNA Restriction Enzymes/metabolism, DNA, Viral/analysis, Ectromelia virus/analysis, Fowlpox virus/analysis, Humans, Infant, Newborn, Molecular Weight, Nucleic Acid Conformation, Poxviridae/analysis, Vaccinia virus/analysis
Pubmed
Web of science
Création de la notice
24/01/2008 11:43
Dernière modification de la notice
03/03/2018 19:35
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