Skin-draining lymph node priming is sufficient to induce sterile immunity against pre-erythrocytic malaria.

Détails

ID Serval
serval:BIB_92D899C6A852
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Skin-draining lymph node priming is sufficient to induce sterile immunity against pre-erythrocytic malaria.
Périodique
EMBO molecular medicine
Auteur(s)
Obeid M., Franetich J.F., Lorthiois A., Gego A., Grüner A.C., Tefit M., Boucheix C., Snounou G., Mazier D.
ISSN
1757-4684 (Electronic)
ISSN-L
1757-4676
Statut éditorial
Publié
Date de publication
02/2013
Peer-reviewed
Oui
Volume
5
Numéro
2
Pages
250-263
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The Plasmodium-infected hepatocyte has been considered necessary to prime the immune responses leading to sterile protection after vaccination with attenuated sporozoites. However, it has recently been demonstrated that priming also occurs in the skin. We wished to establish if sterile protection could be obtained in the absence of priming by infected hepatocytes. To this end, we developed a subcutaneous (s.c.) immunization protocol where few, possibly none, of the immunizing irradiated Plasmodium yoelii sporozoites infect hepatocytes, and also used CD81-deficient mice non-permissive to productive hepatocyte infections. We then compared and contrasted the patterns of priming with those obtained by intradermal immunization, where priming occurs in the liver. Using sterile immunity as a primary read-out, we exploited an inhibitor of T-cell migration, transgenic mice with conditional depletion of dendritic cells and adoptive transfers of draining lymph node-derived T cells, to provide evidence that responses leading to sterile immunity can be primed in the skin-draining lymph nodes with little, if any, contribution from the infected hepatocyte.

Mots-clé
Animals, Erythrocytes/immunology, Erythrocytes/parasitology, Female, Hepatocytes/immunology, Hepatocytes/parasitology, Humans, Immunity, Immunization, Liver/immunology, Lymph Nodes/immunology, Malaria/immunology, Malaria/parasitology, Malaria Vaccines/administration & dosage, Malaria Vaccines/immunology, Mice, Mice, Inbred BALB C, Mice, Transgenic, Plasmodium yoelii/growth & development, Plasmodium yoelii/immunology, Skin/immunology, Skin/parasitology, Sporozoites/growth & development, Sporozoites/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/09/2017 18:28
Dernière modification de la notice
08/05/2019 22:07
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