Proteome analysis of human plasma and amniotic fluid by Off-Gel isoelectric focusing followed by nano-LC-MS/MS

Details

Serval ID
serval:BIB_926B241627CC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Proteome analysis of human plasma and amniotic fluid by Off-Gel isoelectric focusing followed by nano-LC-MS/MS
Journal
Electrophoresis
Author(s)
Michel  P. E., Crettaz  D., Morier  P., Heller  M., Gallot  D., Tissot  J. D., Reymond  F., Rossier  J. S.
ISSN
0173-0835 (Print)
Publication state
Published
Issued date
03/2006
Volume
27
Number
5-6
Pages
1169-81
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
This paper presents a comparative proteomic analysis of human maternal plasma and amniotic fluid (AF) samples from the same patient at term of pregnancy in order to find specific AF proteins as markers of premature rupture of membranes, a complication frequently observed during pregnancy. Maternal plasma and the corresponding AF were immunodepleted in order to remove the six most abundant proteins before the systematic analysis of their protein composition. The protein samples were then fractionated by IEF Off-Gel electrophoresis (OGE), digested and analyzed with nano-LC-MS/MS separation, revealing a total of 73 and 69 proteins identified in maternal plasma and AF samples, respectively. The proteins identified in AF have been compared to those identified in the mother plasma as well as to the reference human plasma protein list reported by Anderson et al. (Mol. Cell. Proteomics 2004, 3, 311-326). This comparison showed that 26 proteins were exclusively present in AF and not in plasma among which 10 have already been described to be placenta or pregnancy specific. As a further validation of the method, plasma proteins fractionated by OGE and analysed by nano-LC-MS/MS have been compared to the Swiss 2-D PAGE reference map by reconstructing a map that matches 2-D gel and OGE experimental data. This representation shows that 36 of 49 reference proteins could be identified in both data sets, and that isoform shifts in pI are well conserved in the OGE data sets.
Keywords
Amniotic Fluid/*chemistry Blood Proteins/*analysis Chromatography, Liquid/methods Electrophoresis, Gel, Two-Dimensional/methods Female Fetal Membranes, Premature Rupture/blood/metabolism Humans Isoelectric Focusing/*methods Mass Spectrometry/*methods Nanotechnology Peptide Mapping Pregnancy Pregnancy Proteins/*analysis Proteome/*analysis
Pubmed
Web of science
Create date
25/01/2008 15:34
Last modification date
20/08/2019 14:55
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