Article: article from journal or magazin.
Rapid death and regeneration of NKT cells in anti-CD3epsilon- or IL-12-treated mice: a major role for bone marrow in NKT cell homeostasis.
Publication types: Journal Article Publication Status: ppublish
Natural killer T (NKT) cells express a T cell receptor (TCR) and markers common to NK cells, including NK1.1. In vivo, NKT cells are triggered by anti-CD3epsilon MAb to rapidly produce large amounts of IL-4 and by IL-12 to reject tumors. We show here that anti-CD3epsilon MAb treatment rapidly depletes the liver (and partially the spleen) of NKT cells and that homeostasis is achieved 1 to 2 days later via NKT cell proliferation that occurs mainly in bone marrow. Similar results were obtained in mice treated with IL-12. Collectively, our data demonstrate that peripheral NKT cells are highly sensitive to activation-induced cell death and that bone marrow plays a major role in restoring NKT cell homeostasis.
Animals, Antibodies, Monoclonal/administration & dosage, Antibodies, Monoclonal/analysis, Antigens/analysis, Antigens, CD3/immunology, Antigens, Ly, Antigens, Surface, Apoptosis, Bone Marrow/physiology, Cell Death/drug effects, Cell Division/drug effects, Female, Homeostasis, Interleukin-12/administration & dosage, Interleukin-12/pharmacology, Killer Cells, Natural/cytology, Lectins, C-Type, Liver/cytology, Lymphocyte Activation, Mice, Mice, Inbred C57BL, NK Cell Lectin-Like Receptor Subfamily B, Proteins/analysis, Spleen/cytology, T-Lymphocytes/cytology, T-Lymphocytes/drug effects, Thymus Gland/cytology, Thymus Gland/physiology
Web of science
Last modification date