Article: article from journal or magazin.
Expression of endothelin-1 in human broncho-epithelial and monocytic cell lines: influence of tumor necrosis factor-alpha and dexamethasone.
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Feb 21
Abnormal endothelin-1 (ET-1) expression has been observed in bronchial asthma and systemic sclerosis with lung involvement. The purpose of this study was to analyze the synthesis of ET-1 in human airway epithelial cells and macrophages under basal conditions and after challenge with tumor necrosis factor-alpha (TNF alpha) or with the glucocorticoid dexamethasone. The ET-1 mRNA level and peptide release were measured in the broncho-epithelial cells BEAS-2B and the monocytic cell line U937. At baseline, U937 cells released low amounts of ET-1 peptide, whereas ET-1 was not detectable in BEAS-2B cells. After TNF alpha treatment, BEAS-2B cells, but not U937 cells, showed a significant increase in ET-1 expression, both at the mRNA and peptide levels. In contrast, dexamethasone elicited an increased amount of ET-1 peptide in U937 medium, but not in BEAS-2B cells. In this latter cell line, dexamethasone pretreatment was unable to inhibit the TNF alpha-induced expression. We conclude that response to TNF alpha and glucocorticoids is cell-type specific with respect to ET-1 production. The response of lung tissue to these agents in vivo is likely to be the overall balance of induction and inhibition in local microenvironments.
Blotting, Northern, Bronchi, Cell Line, Dexamethasone, Endothelin-1, Endothelins, Humans, Monocytes, Protein Precursors, RNA, Messenger, Tumor Necrosis Factor-alpha
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