Expression of endothelin-1 in human broncho-epithelial and monocytic cell lines: influence of tumor necrosis factor-alpha and dexamethasone.

Détails

ID Serval
serval:BIB_903110AF11FD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expression of endothelin-1 in human broncho-epithelial and monocytic cell lines: influence of tumor necrosis factor-alpha and dexamethasone.
Périodique
Biochemical Pharmacology
Auteur(s)
Aubert J.D., Juillerat-Jeanneret L., Leuenberger P.
ISSN
0006-2952
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
53
Numéro
4
Pages
547-552
Langue
anglais
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Feb 21
Résumé
Abnormal endothelin-1 (ET-1) expression has been observed in bronchial asthma and systemic sclerosis with lung involvement. The purpose of this study was to analyze the synthesis of ET-1 in human airway epithelial cells and macrophages under basal conditions and after challenge with tumor necrosis factor-alpha (TNF alpha) or with the glucocorticoid dexamethasone. The ET-1 mRNA level and peptide release were measured in the broncho-epithelial cells BEAS-2B and the monocytic cell line U937. At baseline, U937 cells released low amounts of ET-1 peptide, whereas ET-1 was not detectable in BEAS-2B cells. After TNF alpha treatment, BEAS-2B cells, but not U937 cells, showed a significant increase in ET-1 expression, both at the mRNA and peptide levels. In contrast, dexamethasone elicited an increased amount of ET-1 peptide in U937 medium, but not in BEAS-2B cells. In this latter cell line, dexamethasone pretreatment was unable to inhibit the TNF alpha-induced expression. We conclude that response to TNF alpha and glucocorticoids is cell-type specific with respect to ET-1 production. The response of lung tissue to these agents in vivo is likely to be the overall balance of induction and inhibition in local microenvironments.
Mots-clé
Blotting, Northern, Bronchi, Cell Line, Dexamethasone, Endothelin-1, Endothelins, Humans, Monocytes, Protein Precursors, RNA, Messenger, Tumor Necrosis Factor-alpha
Pubmed
Web of science
Création de la notice
25/01/2008 10:50
Dernière modification de la notice
03/03/2018 19:24
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