Article: article from journal or magazin.
Astrocytes secrete a factor inducing the expression of HT7-protein and neurothelin in endothelial cells of chorioallantoic vessels
Advances in Experimental Medicine and Biology
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Among the most specific markers of the blood-brain barrier phenotype of endothelial cells are the well-characterized immunoglobulin-like surface glycoprotein HT7 and the probably related or identical glycoprotein neurothelin. Both can be induced in chorioallantoic vessels by transplants of embryonic mouse brain. Other blood-brain barrier markers have been shown to be inducible by type-1 astrocytes in endothelial cells of non-neural origin. In the present work we tested the hypothesis that this cellular interaction between astrocytes and endothelial cells is mediated by a soluble factor(s). Chorioallantoic vessels of embryonic day 9 chick embryos were exposed for 4 or 10 days to a constant and localized delivery of astrocyte-conditioned medium by using a piece of gelfoam posed onto the chorioallantoic membrane, as a localized reservoir, which was connected to a miniosmotic pump system delivering astrocyte-conditioned medium at a steady rate. We found that in a significant number of chorioallantoic vessels located near the gelfoam, endothelial cells exposed to astrocyte-conditioned medium for a period of 4 or 10 days, but not to glioma-, fibroblast- or endothelial cell-derived conditioned medium, expressed the HT7 antigen and neurothelin. These results provide evidence that type-1-astrocytes are capable of inducing blood-brain barrier related properties in endothelial cells of non-neural origin through a soluble factor(s)
Allantois/Animals/Antigens,CD/Antigens,CD147/Antigens,Neoplasm/Antigens,Surface/Astrocytes/Avian Proteins/Biological Factors/biosynthesis/Blood Proteins/blood supply/Blood-Brain Barrier/Brain/Cells/Cells,Cultured/Chick Embryo/Chorion/cytology/Embryo/Endothelial Cells/Endothelium,Vascular/Glioma/Membrane Glycoproteins/metabolism/Pathology/Phenotype/physiology/Research/secretion/Solubility/Switzerland
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